Titre
Holliday junction branch migration and resolution assays
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Methods in Molecular Biology
Auteur(s)
Constantinou, A.
Auteure/Auteur
West, S. C.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1064-3745
Statut éditorial
Publié
Date de publication
2004
Volume
262
Première page
239
Dernière page/numéro d’article
53
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Résumé
Holliday junctions are central intermediates in the process of genetic recombination; they form as a consequence of a reciprocal exchange of strands between paired DNA molecules. Enzymes that specifically recognize and process these junctions are necessary for the formation of recombinant products. In the methods described here, we detail the in vitro construction of two types of Holliday junction: (1) a small synthetic junction formed by the annealing of partially complementary oligonucleotides; and (2) a true recombination intermediate structure formed by RecA protein-mediated strand exchange. The use of these substrates in assays designed to detect Holliday junction branch migration and resolution activities is described.
Sujets
PID Serval
serval:BIB_65934DB80F25
PMID
Date de création
2008-01-24T13:50:56.985Z
Date de création dans IRIS
2025-05-21T03:34:29Z