Titre
The Cockayne syndrome B protein, involved in transcription-coupled DNA repair, resides in an RNA polymerase II-containing complex
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
van Gool, A. J.
Auteure/Auteur
Citterio, E.
Auteure/Auteur
Rademakers, S.
Auteure/Auteur
van Os, R.
Auteure/Auteur
Vermeulen, W.
Auteure/Auteur
Constantinou, A.
Auteure/Auteur
Egly, J. M.
Auteure/Auteur
Bootsma, D.
Auteure/Auteur
Hoeijmakers, J. H.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0261-4189
Statut éditorial
Publié
Date de publication
1997-10
Volume
16
Numéro
19
Première page
5955
Dernière page/numéro d’article
65
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 1
Research Support, Non-U.S. Gov't --- Old month value: Oct 1
Résumé
Transcription-coupled repair (TCR), a subpathway of nucleotide excision repair (NER) defective in Cockayne syndrome A and B (CSA and CSB), is responsible for the preferential removal of DNA lesions from the transcribed strand of active genes, permitting rapid resumption of blocked transcription. Here we demonstrate by microinjection of antibodies against CSB and CSA gene products into living primary fibroblasts, that both proteins are required for TCR and for recovery of RNA synthesis after UV damage in vivo but not for basal transcription itself. Furthermore, immunodepletion showed that CSB is not required for in vitro NER or transcription. Its central role in TCR suggests that CSB interacts with other repair and transcription proteins. Gel filtration of repair- and transcription-competent whole cell extracts provided evidence that CSB and CSA are part of large complexes of different sizes. Unexpectedly, there was no detectable association of CSB with several candidate NER and transcription proteins. However, a minor but significant portion (10-15%) of RNA polymerase II was found to be tightly associated with CSB. We conclude that within cell-free extracts, CSB is not stably associated with the majority of core NER or transcription components, but is part of a distinct complex involving RNA polymerase II. These findings suggest that CSB is implicated in, but not essential for, transcription, and support the idea that Cockayne syndrome is due to a combined repair and transcription deficiency.
Sujets
PID Serval
serval:BIB_5221B8EB0D97
PMID
Open Access
Oui
Date de création
2008-01-24T13:50:42.399Z
Date de création dans IRIS
2025-05-20T18:34:01Z