Titre
Does wheat genetically modified for disease resistance affect root-colonizing pseudomonads and arbuscular mycorrhizal fungi?
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Meyer, J.B.
Auteure/Auteur
Song-Wilson, Y.
Auteure/Auteur
Foetzki, A.
Auteure/Auteur
Luginbühl, C.
Auteure/Auteur
Winzeler, M.
Auteure/Auteur
Kneubühler, Y.
Auteure/Auteur
Matasci, C.
Auteure/Auteur
Mascher-Frutschi, F.
Auteure/Auteur
Kalinina, O.
Auteure/Auteur
Boller, T.
Auteure/Auteur
Keel, C.
Co-dernière auteure/Co-dernier auteur
Maurhofer, M.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1932-6203
Statut éditorial
Publié
Date de publication
2013
Volume
8
Numéro
1
Première page
e53825
Langue
anglais
Résumé
This study aimed to evaluate the impact of genetically modified (GM) wheat with introduced pm3b mildew resistance transgene, on two types of root-colonizing microorganisms, namely pseudomonads and arbuscular mycorrhizal fungi (AMF). Our investigations were carried out in field trials over three field seasons and at two locations. Serial dilution in selective King's B medium and microscopy were used to assess the abundance of cultivable pseudomonads and AMF, respectively. We developed a denaturing gradient gel electrophoresis (DGGE) method to characterize the diversity of the pqqC gene, which is involved in Pseudomonas phosphate solubilization. A major result was that in the first field season Pseudomonas abundances and diversity on roots of GM pm3b lines, but also on non-GM sister lines were different from those of the parental lines and conventional wheat cultivars. This indicates a strong effect of the procedures by which these plants were created, as GM and sister lines were generated via tissue cultures and propagated in the greenhouse. Moreover, Pseudomonas population sizes and DGGE profiles varied considerably between individual GM lines with different genomic locations of the pm3b transgene. At individual time points, differences in Pseudomonas and AMF accumulation between GM and control lines were detected, but they were not consistent and much less pronounced than differences detected between young and old plants, different conventional wheat cultivars or at different locations and field seasons. Thus, we conclude that impacts of GM wheat on plant-beneficial root-colonizing microorganisms are minor and not of ecological importance. The cultivation-independent pqqC-DGGE approach proved to be a useful tool for monitoring the dynamics of Pseudomonas populations in a wheat field and even sensitive enough for detecting population responses to altered plant physiology.
PID Serval
serval:BIB_8CA2F788D83B
PMID
Open Access
Oui
Date de création
2011-10-20T10:04:49.313Z
Date de création dans IRIS
2025-05-20T21:33:25Z
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Nom
BIB_8CA2F788D83B.P001.pdf
Version du manuscrit
preprint
Taille
1.08 MB
Format
Adobe PDF
PID Serval
serval:BIB_8CA2F788D83B.P001
URN
urn:nbn:ch:serval-BIB_8CA2F788D83B3
Somme de contrôle
(MD5):8edf405ecf39755be033bd51fb1bbf00