Titre
Soft X-irradiation influences the integrity of Langerhans cells. A histochemical and immunohistological study
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Groh, V.
Auteure/Auteur
Meyer, J. C.
Auteure/Auteur
Panizzon, R.
Auteure/Auteur
Zortea-Caflisch, C.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0011-9075
Statut éditorial
Publié
Date de publication
1984
Volume
168
Numéro
2
Première page
53
Dernière page/numéro d’article
60
Notes
Journal Article
Résumé
This report defines the influence of field soft X-ray irradiation on the integrity of epidermal Langerhans cells (LC) in the guinea pig and mouse systems. Male albino (Rockefeller strain) and piebald F 1 (2 X 13 strain) guinea pigs as well as C3H mice (H-2Kk) were exposed to one single shot of different dosages of soft X-rays [80, 1,200, 1,600, and 3,200 R; 30 kV, 0.5 mm aluminium filter, FSD (focus-skin distance) 12 cm]. 1 and 4 weeks after exposure, skin specimens were taken from the irradiated skin. The demonstration and evaluation of LC was performed basing on their expression of specific histochemical (ATPase) and functional immunologic markers (Ia antigens). Soft X-irradiation had pronounced effects on number and structure of ATPase- and Ia-antigen-positive cells. In the mouse system, 1 week after exposure to 800 and 1,200 R ATPase-positive cells and, in a more or less parellel manner, Ia-antigen-positive cells () were reduced to 74% (74%) and 70% (68%), respectively, and 4 weeks after exposure to 49% (47%) and 43% (40%), respectively. In the guinea pig system one single shot of 1,600 or 3,200 R produced, respectively, a 35% or a 38% reduction of ATPase-positive cells. Prolonged survival did not result in a further depletion of ATPase-positive cells.
Sujets
PID Serval
serval:BIB_B4A6CCE2FBC3
PMID
Date de création
2008-01-25T15:54:42.453Z
Date de création dans IRIS
2025-05-21T04:25:20Z