Titre
Measurements by fluorescence microscopy of the time-dependent distribution of meso-tetra-hydroxyphenylchlorin in healthy tissues and chemically induced ''early'' squamous cell carcinoma of the Syrian hamster cheek pouch
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Andrejevic, S.
Auteure/Auteur
Savary, J. F.
Auteure/Auteur
Monnier, P.
Auteure/Auteur
Fontolliet, C.
Auteure/Auteur
Braichotte, D.
Auteure/Auteur
Wagnieres, G.
Auteure/Auteur
vandenBergh, H.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1011-1344
Statut éditorial
Publié
Date de publication
1996-11
Volume
36
Numéro
2
Première page
143
Dernière page/numéro d’article
51
Peer-reviewed
Oui
Résumé
The influence of the time interval between dye administration and detection by fluorescence microscopy was assessed in "early" squamous cell carcinomas of the cheek pouch mucosa and different healthy tissues of the Syrian hamster. Following intracardiac injection of 0.15 mg (kg bodyweight)-1 of meso-tetra-hydroxyphenylchlorin (m-THPC), groups of three animals were sacrificed at different time intervals up to 30 days. A group of three non-injected animals was used to detect the endogene fluorescence of the corresponding normal tissues for autofluorescence subtraction. The following excised organs (oesophagus, trachea, liver, spleen, kidney, skin, striated muscle, healthy and tumoral cheek pouch mucosae) were fast frozen in liquid nitrogen and stored at -70 degrees C for fluorescence microscopy. The results show significant differences in the detectable m-THPC levels in different tissue layers (for instance, the epithelia and muscle of the oesophagus, trachea and cheek pouch) at different time intervals. These data indicate that pharmacokinetic studies may be useful for selecting the optimal time for the photodetection and phototherapy of cancer.
Sujets
PID Serval
serval:BIB_B6504BC4CBA3
PMID
Date de création
2008-12-14T08:03:30.578Z
Date de création dans IRIS
2025-05-20T23:17:20Z