Titre
Haemodialysis acutely reduces the plasma levels of ADMA without reversing impaired NO-dependent vasodilation.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Engelberger, R.P.
Auteure/Auteur
Teta, D.
Auteure/Auteur
Henry, H.
Auteure/Auteur
De Senarclens, O.
Auteure/Auteur
Dischl, B.
Auteure/Auteur
Liaudet, L.
Auteure/Auteur
Burnier, M.
Auteure/Auteur
Waeber, B.
Auteure/Auteur
Feihl, F.
Auteure/Auteur
Liens vers les personnes
ISSN
1470-8736[electronic]
Statut éditorial
Publié
Date de publication
2009
Volume
117
Numéro
7-8
Première page
293
Dernière page/numéro d’article
303
Langue
anglais
Résumé
End-stage renal disease patients have endothelial dysfunction and high plasma levels of ADMA (asymmetric omega-NG,NG-dimethylarginine), an endogenous inhibitor of NOS (NO synthase). The actual link between these abnormalities is controversial. Therefore, in the present study, we investigated whether HD (haemodialysis) has an acute impact on NO-dependent vasodilation and plasma ADMA in these patients. A total of 24 patients undergoing maintenance HD (HD group) and 24 age- and gender-matched healthy controls (Control group) were enrolled. The increase in forearm SkBF (skin blood flow) caused by local heating to 41 degrees C (SkBF41), known to depend on endothelial NO production, was determined with laser Doppler imaging. SkBF41 was expressed as a percentage of the vasodilatory reserve obtained from the maximal SkBF induced by local heating to 43 degrees C (independent of NO). In HD patients, SkBF41 was assessed on two successive HD sessions, once immediately before and once immediately after HD. Plasma ADMA was assayed simultaneously with MS/MS (tandem MS). In the Control group, SkBF41 was determined twice, on two different days, and plasma ADMA was assayed once. In HD patients, SkBF41 was identical before (82.2+/-13.1%) and after (82.7+/-12.4%) HD, but was lower than in controls (day 1, 89.6+/-6.1; day 2, 89.2+/-6.9%; P<0.01 compared with the HD group). In contrast, plasma ADMA was higher before (0.98+/-0.17 micromol/l) than after (0.58+/-0.10 micromol/l; P<0.01) HD. ADMA levels after HD did not differ from those obtained in controls (0.56+/-0.11 micromol/l). These findings show that HD patients have impaired NO-dependent vasodilation in forearm skin, an abnormality not acutely reversed by HD and not explained by ADMA accumulation.
Sujets
PID Serval
serval:BIB_DB929680ECA0
PMID
Open Access
Oui
Date de création
2009-10-29T08:47:55.609Z
Date de création dans IRIS
2025-05-21T02:59:56Z
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Nom
BIB_DB929680ECA0.P001.pdf
Version du manuscrit
published
Taille
267.24 KB
Format
Adobe PDF
PID Serval
serval:BIB_DB929680ECA0.P001
URN
urn:nbn:ch:serval-BIB_DB929680ECA03
Somme de contrôle
(MD5):70bced7cba0fba4319f438d3ff140930