Titre
Stiffness tomography exploration of living and fixed macrophages.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Roduit, C.
Auteure/Auteur
Longo, G.
Auteure/Auteur
Benmessaoud, I.
Auteure/Auteur
Volterra, A.
Auteure/Auteur
Saha, B.
Auteure/Auteur
Dietler, G.
Auteure/Auteur
Kasas, S.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1099-1352
Statut éditorial
Publié
Date de publication
2012
Volume
25
Numéro
5
Première page
241
Dernière page/numéro d’article
246
Peer-reviewed
Oui
Langue
anglais
Résumé
Stiffness tomography is a new atomic force microscopy imaging technique that allows highlighting structures located underneath the surface of the sample. In this imaging mode, such structures are identified by investigating their mechanical properties. We present here, for the first time, a description of the use of this technique to acquire detailed stiffness maps of fixed and living macrophages. Indeed, the mechanical properties of several macrophages were studied through stiffness tomography imaging, allowing some insight of the structures lying below the cell's surface. Through these investigations, we were able to evidence the presence and properties of stiff column-like features located underneath the cell membrane. To our knowledge, this is the first evidence of the presence, underneath the cell membrane, of such stiff features, which are in dimension and form compatible with phagosomes. Moreover, by exposing the cells to cytochalasin, we were able to study the induced modifications, obtaining an indication of the location and mechanical properties of the actin cytoskeleton. Copyright © 2012 John Wiley & Sons, Ltd.
PID Serval
serval:BIB_3B14E4D048B8
PMID
Date de création
2012-05-23T09:15:09.203Z
Date de création dans IRIS
2025-05-20T20:45:22Z