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  4. Interleukin-17E, inducible nitric oxide synthase and arginase1 as new biomarkers in the identification of neutrophilic dermatoses.
 
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Titre

Interleukin-17E, inducible nitric oxide synthase and arginase1 as new biomarkers in the identification of neutrophilic dermatoses.

Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Clinical and Experimental Dermatology  
Auteur(s)
Stalder, R.
Auteure/Auteur
Brembilla, N.
Auteure/Auteur
Conrad, C.
Auteure/Auteur
Yawalkar, N.
Auteure/Auteur
Navarini, A.
Auteure/Auteur
Boehncke, W.H.
Auteure/Auteur
Kaya, G.
Auteure/Auteur
Liens vers les personnes
Conrad, Curdin  
Liens vers les unités
Dermatologie  
ISSN
1365-2230
Statut éditorial
Publié
Date de publication
2022-04
Volume
47
Numéro
4
Première page
675
Dernière page/numéro d’article
683
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Neutrophilic dermatoses (ND) are a heterogeneous group of diseases, but can often have a relatively similar histological appearance.
To identify a combination of biomarkers allowing a better differentiation of ND types.
Biopsies were obtained from normal human skin (NS; n = 4), chronic plaque-type psoriasis (PsO; n = 7), paradoxical psoriasis (PP; n = 8), generalized pustular psoriasis (GPP; n = 9), subcorneal pustular dermatosis of Sneddon-Wilkinson (SPD; n = 3), acute generalized exanthematous pustulosis (AGEP; n = 3), hidradenitis suppurativa (HS; n = 7), Sweet syndrome (SS; n = 8) and pyoderma gangrenosum (PG; n = 8). Samples were analysed by immunofluorescence using three biomarkers, interleukin (IL)-17E, inducible nitric oxide synthase (iNOS) and arginase1, each one in combination with two cell markers, myeloperoxidase (MPO) and CD68, which allow the identification of neutrophils and macrophages, respectively.
We found that SS is characterized by high expression of IL-17E and iNOS in the epidermis, while PG exhibits low expression. The density of the neutrophil infiltrate helps to differentiate PP (high-density infiltrate) from PsO (low-density infiltrate). High expression of arginase1 in the granular layer of the epidermis is a hallmark of SPD. Finally, mature neutrophils and proinflammatory macrophages are readily detectable in PP, SPD and PG, whereas immature neutrophils and anti-inflammatory macrophages are more frequent in GPP, AGEP, HS and SS.
The analysis of ND by immunofluorescence using IL-17E, iNOS and arginase1 in combination with MPO and CD68 allows for characterization of differential expression patterns in the epidermis as well as the determination of the polarization status of the dermal neutrophils and macrophages. The appropriate markers may help in the differentiation of ND in clinical practice.
Sujets

Arginase

Biomarkers

Dermatitis/diagnosis

Dermatitis/pathology

Humans

Interleukin-17

Nitric Oxide Synthase...

Psoriasis/diagnosis

Psoriasis/pathology

PID Serval
serval:BIB_A648075F44ED
DOI
10.1111/ced.14988
PMID
34669971
WOS
000731190300001
Permalien
https://iris.unil.ch/handle/iris/135267
Open Access
Oui
Date de création
2021-10-25T07:05:28.040Z
Date de création dans IRIS
2025-05-20T21:14:55Z
Fichier(s)
En cours de chargement...
Vignette d'image
Nom

34669971_BIB_A648075F44ED.pdf

Version du manuscrit

preprint

Licence

https://creativecommons.org/licenses/by-nc-nd/4.0

Taille

12.73 MB

Format

Adobe PDF

PID Serval

serval:BIB_A648075F44ED.P001

URN

urn:nbn:ch:serval-BIB_A648075F44ED3

Somme de contrôle

(MD5):b1fda77959a213ef9a897a6a3c032c73

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