Titre
Analysis of enantiomers of citalopram and its demethylated metabolites in plasma of depressive patients using chiral reverse-phase liquid chromatography.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Rochat, B.
Auteure/Auteur
Amey, M.
Auteure/Auteur
Baumann, P.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0163-4356
Statut éditorial
Publié
Date de publication
1995-06
Volume
17
Numéro
3
Première page
273
Dernière page/numéro d’article
279
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
A stereospecific high-performance liquid chromatography (HPLC) method has been developed for the analysis of the underived enantiomers of citalopram (CIT) and its N-demethylated metabolites in plasma. Using fluorescence detection, the limit of quantification for each enantiomer is 3 ng/ml. CIT N-oxide and the CIT propionic acid derivative are not extracted by the procedure used. Inter- and intraday validations of the method using reverse-phase mode HPLC on separate acetylated beta-cyclobond columns showed the sensitivity of this assay to be suitable for pharmacokinetic studies of the enantiomers of these compounds. Plasma levels of the enantiomers and the demethylated metabolites of CIT have been determined during routine therapeutic drug monitoring (TDM) in 29 depressive patients treated with varying dosages (20-80 mg/day) of CIT. Concentrations of S-(+)-CIT, which is considered the most potent selective serotonin reuptake inhibitors (SSRI) of the CIT and desmethylcitalopram (DCIT) enantiomers, varied between 24-49% (mean +/- sd, 35% +/- 5%) of the concentrations of total CIT. There were highly significant correlations between S-(+)-CIT and R-(-)-CIT levels (r = 0.866; p < 0.0001) and between S-(+)-DCIT and R-(-)-CIT (r = 0.932; p < 0.0001). The co-medications seemed to have little influence on enantiomer ratios. These results suggest the need for studies on the relationships between clinical response and plasma levels of CIT enantiomers.
PID Serval
serval:BIB_C37223FD6767
PMID
Date de création
2008-01-25T07:47:08.744Z
Date de création dans IRIS
2025-05-20T21:52:03Z