Titre
Three ATPase activities have an abnormal developmental time course in trembler sciatic nerves
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Burgisser, P.
Auteure/Auteur
Geering, K.
Auteure/Auteur
Rossier, B. C.
Auteure/Auteur
Matthieu, J. M.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0378-5866
Statut éditorial
Publié
Date de publication
1987
Volume
9
Numéro
1
Première page
45
Dernière page/numéro d’article
52
Peer-reviewed
Oui
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Résumé
Trembler mutant mice are affected by a peripheral neuropathy characterized by hypomyelination, demyelination, and Schwann cell proliferation. In adult mutants, supernumerary Schwann cells form membranous structures known as 'onion-bulb' formations. The activities of the Na+, K+-ATPase and of two ouabain-insensitive Mg2+-ATPases were investigated in sciatic nerves of young and adult mutants. The Na+, K+-ATPase activities were 92 and 76% of the control values in young and adult mutants, respectively. By immunoblot analysis, the alpha-subunit of the Na+, K+-ATPase had an identical apparent molecular weight in controls at both ages and in young mutants. In adult mutants, on the contrary, the alpha-subunit appeared smaller by about 2 kd, similar to that in kidney, indicating that the Na+, K+-ATPase was localized mainly on supernumerary Schwann cells. In addition, in the mutants, the developmental increase of both the mitochondrial and the nonmitochondrial Mg2+-ATPase was abnormally high. We suggest that the abnormal increase of the nonmitochondrial Mg2+-ATPase activity during development reflects an enrichment of that enzyme in 'onion-bulb' formations.
Sujets
PID Serval
serval:BIB_991481BB7051
PMID
Date de création
2008-01-24T12:01:11.426Z
Date de création dans IRIS
2025-05-20T22:06:11Z