Titre
Genome-wide search reveals a novel GacA-regulated small RNA in Pseudomonas species.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
González, N.
Auteure/Auteur
Heeb, S.
Auteure/Auteur
Valverde, C.
Auteure/Auteur
Kay, E.
Auteure/Auteur
Reimmann, C.
Auteure/Auteur
Junier, T.
Auteure/Auteur
Haas, D.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1471-2164
Statut éditorial
Publié
Date de publication
2008
Volume
9
Première page
167
Peer-reviewed
Oui
Langue
anglais
Résumé
BACKGROUND: Small RNAs (sRNAs) are widespread among bacteria and have diverse regulatory roles. Most of these sRNAs have been discovered by a combination of computational and experimental methods. In Pseudomonas aeruginosa, a ubiquitous Gram-negative bacterium and opportunistic human pathogen, the GacS/GacA two-component system positively controls the transcription of two sRNAs (RsmY, RsmZ), which are crucial for the expression of genes involved in virulence. In the biocontrol bacterium Pseudomonas fluorescens CHA0, three GacA-controlled sRNAs (RsmX, RsmY, RsmZ) regulate the response to oxidative stress and the expression of extracellular products including biocontrol factors. RsmX, RsmY and RsmZ contain multiple unpaired GGA motifs and control the expression of target mRNAs at the translational level, by sequestration of translational repressor proteins of the RsmA family. RESULTS: A combined computational and experimental approach enabled us to identify 14 intergenic regions encoding sRNAs in P. aeruginosa. Eight of these regions encode newly identified sRNAs. The intergenic region 1698 was found to specify a novel GacA-controlled sRNA termed RgsA. GacA regulation appeared to be indirect. In P. fluorescens CHA0, an RgsA homolog was also expressed under positive GacA control. This 120-nt sRNA contained a single GGA motif and, unlike RsmX, RsmY and RsmZ, was unable to derepress translation of the hcnA gene (involved in the biosynthesis of the biocontrol factor hydrogen cyanide), but contributed to the bacterium's resistance to hydrogen peroxide. In both P. aeruginosa and P. fluorescens the stress sigma factor RpoS was essential for RgsA expression. CONCLUSION: The discovery of an additional sRNA expressed under GacA control in two Pseudomonas species highlights the complexity of this global regulatory system and suggests that the mode of action of GacA control may be more elaborate than previously suspected. Our results also confirm that several GGA motifs are required in an sRNA for sequestration of the RsmA protein.
PID Serval
serval:BIB_B3ADE80624C0
PMID
Open Access
Oui
Date de création
2009-01-29T21:13:13.367Z
Date de création dans IRIS
2025-05-20T22:13:50Z
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Nom
BIB_B3ADE80624C0.P001.pdf
Version du manuscrit
preprint
Taille
832.67 KB
Format
Adobe PDF
PID Serval
serval:BIB_B3ADE80624C0.P001
URN
urn:nbn:ch:serval-BIB_B3ADE80624C02
Somme de contrôle
(MD5):5626b5a6bf719144fc89d3dcb684221b