Titre
High- and low-affinity binding sites for vasoactive intestinal peptide (VIP) in the rat kidney revealed by light microscopic autoradiography
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Regulatory Peptides
Auteur(s)
Magistretti, P. J.
Auteure/Auteur
Hof, P. R.
Auteure/Auteur
Martin, J. L.
Auteure/Auteur
Dietl, M.
Auteure/Auteur
Palacios, J. M.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0167-0115
Statut éditorial
Publié
Date de publication
1988-11
Volume
23
Numéro
2
Première page
145
Dernière page/numéro d’article
52
Peer-reviewed
Oui
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
The distribution of VIP binding sites in rat kidney and adrenal gland has been examined by light microscopic autoradiography. A fully characterized mono-iodinated molecular form of VIP (M-125-I-VIP) which maintains the biological activity of the native peptide, was used for this study. Two types of VIP binding sites, with high and low affinity, have been identified. High affinity sites are associated with (i) glomerular structures in the cortex, (ii) the inner stripe of the outer medulla, possibly corresponding to Henle's loops and distal tubules, (iii) radiated structures in the inner zone of the medulla, likely to represent labeling of collecting ducts and/or vascular bundles and (iv) the adrenal cortex. Autoradiographic grains associated with low affinity sites are present diffusely throughout the renal cortex, possibly corresponding to labeling of tubular and/or vascular structures, and throughout the adrenal gland. These observations further delineate a role of VIP in renal and neuroendocrine function.
Sujets
PID Serval
serval:BIB_A880E260619C
PMID
Date de création
2008-01-20T17:22:15.630Z
Date de création dans IRIS
2025-05-20T22:43:28Z