Titre
Interferon-gamma-treated renal tubular epithelial cells induce allospecific tolerance
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Frasca, L.
Auteure/Auteur
Marelli-Berg, F.
Auteure/Auteur
Imami, N.
Auteure/Auteur
Potolicchio, I.
Auteure/Auteur
Carmichael, P.
Auteure/Auteur
Lombardi, G.
Auteure/Auteur
Lechler, R.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0085-2538
Statut éditorial
Publié
Date de publication
1998-03
Volume
53
Numéro
3
Première page
679
Dernière page/numéro d’article
89
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
Following organ transplantation, tissue parenchymal cells commonly express major histocompatibility complex (MHC) class II molecules as a result of local cytokine release, and thus acquire the capacity to present donor MHC alloantigens to alloreactive CD4+ T cells. The consequences of such a presentation are likely to be relevant in the induction of tolerance to the transplanted tissues, and this has been reported in animal models of transplantation and in humans. In this study, the consequences of antigen presentation by interferon-gamma (IFN-gamma)-treated human renal tubular epithelial cells (RTEC) to resting and activated CD4+ T cells were investigated. Allogeneic RTEC were unable to stimulate proliferation by peripheral blood CD45 RA+ or RO+ CD4+ T cells from three HLA-mismatched responders. The response to RTEC was partially reconstituted by the addition of murine L cell transfectants expressing human B7.1 (DAP.3-B7), suggesting that the failure of RTEC to stimulate a primary alloresponse was due, at least in part, to a lack of costimulation. T cell clones dependent on B7-mediated co-stimulation also did not respond to peptide presented by RTEC. Most importantly, this lack of reactivity was accompanied by the induction of nonresponsiveness. Incubation with allogeneic, DR-expressing RTEC induced allospecific hyporesponsiveness in both CD45RA+ and RO+ cells. Similarly, overnight incubation with antigen-pulsed RTEC induced nonresponsiveness in the B7-dependent T cell clones. These results suggest that MHC class II expression on RTEC may contribute to the induction of an allospecific nonresponsiveness following organ transplantation.
Sujets
PID Serval
serval:BIB_C70918E6EBF6
PMID
Open Access
Oui
Date de création
2008-01-25T14:16:06.506Z
Date de création dans IRIS
2025-05-20T23:16:27Z