Titre
Restoration of growth potential in paraclones of human keratinocytes by a viral oncogene
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Barrandon, Y.
Auteure/Auteur
Morgan, J. R.
Auteure/Auteur
Mulligan, R. C.
Auteure/Auteur
Green, H.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0027-8424
Statut éditorial
Publié
Date de publication
1989-06
Volume
86
Numéro
11
Première page
4102
Dernière page/numéro d’article
6
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jun
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jun
Résumé
Human diploid keratinocytes may be divided into three clonal types with differing capacities for proliferation. The paraclone, which has the shortest life span, is limited to 15 divisions, after which all the cells undergo programmed terminal differentiation. By means of a retroviral vector, paraclones which have not completed their life span and which consist of not more than a few hundred cells can be transduced at a high frequency with DNA complementary to the 12S transcript of the adenovirus early region 1A gene. Transformation can be detected within a single cultivation by the formation of progressively growing colonies. The transformants appear to have an unlimited growth potential, and they form a disorganized epidermis when they are grafted as an epithelium onto athymic mice. These experiments clearly show that, in order to be transformed by a viral oncogene, the target cell need not be a stem cell.
Sujets
PID Serval
serval:BIB_B95747560B32
PMID
Open Access
Oui
Date de création
2008-01-28T07:41:48.335Z
Date de création dans IRIS
2025-05-20T23:21:15Z