Titre
Measurement of immunoreactive angiotensin-(1-7) heptapeptide in human blood.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Nussberger, J.
Auteure/Auteur
Brunner, D.B.
Auteure/Auteur
Nyfeler, J.A.
Auteure/Auteur
Linder, L.
Auteure/Auteur
Brunner, H.R.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0009-9147
Statut éditorial
Publié
Date de publication
2001
Volume
47
Numéro
4
Première page
726
Dernière page/numéro d’article
9
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article - Publication Status: ppublish
Résumé
BACKGROUND: The renal enzyme renin cleaves from the hepatic alpha(2)-globulin angiotensinogen angiotensin-(1-10) decapeptide [Ang-(1-10)], which is further metabolized to smaller peptides that help maintain cardiovascular homeostasis. The Ang-(1-7) heptapeptide has been reported to have several physiological effects, including natriuresis, diuresis, vasodilation, and release of vasopressin and prostaglandins. METHODS: To investigate Ang-(1-7) in clinical settings, we developed a method to measure immunoreactive (ir-) Ang-(1-7) in 2 mL of human blood and to estimate plasma concentrations by correcting for the hematocrit. A sensitive and specific antiserum against Ang-(1-7) was raised in a rabbit. Human blood was collected in the presence of an inhibitor mixture including a renin inhibitor to prevent peptide generation in vitro. Ang-(1-7) was extracted into ethanol and purified on phenylsilylsilica. The peptide was quantified by radioimmunoassay. Increasing doses of Ang-(1-7) were infused into volunteers, and plasma concentrations of the peptide were measured. RESULTS: The detection limit for plasma ir-Ang-(1-7) was 1 pmol/L. CVs for high and low blood concentrations were 4% and 20%, respectively, and between-assay CVs were 8% and 13%, respectively. Reference values for human plasma concentrations of ir-Ang-(1-7) were 1.0-9.5 pmol/L (median, 4.7 pmol/L) and increased linearly during infusion of increasing doses of Ang-(1-7). CONCLUSIONS: Reliable measurement of plasma ir-Ang-(1-7) is achieved with efficient inhibition of enzymes that generate or metabolize Ang-(1-7) after blood sampling, extraction in ethanol, and purification on phenylsilylsilica, and by use of a specific antiserum.
PID Serval
serval:BIB_B58CFB2CE5A5
PMID
Date de création
2008-03-05T15:40:48.145Z
Date de création dans IRIS
2025-05-20T23:45:18Z