Titre
(TG)n uncovers a sex-specific hybridization pattern in cattle
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Kashi, Y.
Auteure/Auteur
Iraqi, F.
Auteure/Auteur
Tikochinski, Y.
Auteure/Auteur
Ruzitsky, B.
Auteure/Auteur
Nave, A.
Auteure/Auteur
Beckmann, J. S.
Auteure/Auteur
Friedmann, A.
Auteure/Auteur
Soller, M.
Auteure/Auteur
Gruenbaum, Y.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0888-7543
Statut éditorial
Publié
Date de publication
1990-05
Volume
7
Numéro
1
Première page
31
Dernière page/numéro d’article
6
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: May
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: May
Résumé
Screening of a bovine genomic library with the human minisatellite 33.6 probe uncovered a family of clones that, when used to probe Southern blots of bovine genomic DNA digested with the restriction enzyme HaeIII or MboI, revealed sexually dimorphic, but otherwise virtually monomorphic, patterns among the larger DNA fragments to which they hybridized. Characterization of one of these clones revealed that it contains different minisatellite sequences. The sexual dimorphism hybridization pattern observed with this clone was found to be due to multiple copies of two tandemly interspersed repeats: the simple sequence (TG)n and a previously undescribed 29-bp sequence. Both repeats appear to share many genomic loci including autosomal loci. In contrast, Southern analysis of AluI- or HinfI-digested bovine DNA with the (TG)n repeat used as a probe yielded substantial polymorphism. These results show that (i) different minisatellites can be found in a cluster, (ii) both simple and more complex repeated sequences other than the simple quaternary (GATA)n repeat can be sexually dimorphic, and (iii) simple repeats can reveal substantial polymorphism.
Sujets
PID Serval
serval:BIB_6677E86A54D1
PMID
Date de création
2008-01-25T15:18:36.231Z
Date de création dans IRIS
2025-05-21T00:24:16Z