Titre
The aldosterone-mineralocorticoid receptor pathway exerts anti-inflammatory effects in endotoxin-induced uveitis.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Bousquet, E.
Auteure/Auteur
Zhao, M.
Auteure/Auteur
Ly, A.
Auteure/Auteur
Leroux Les Jardins, G.
Auteure/Auteur
Goldenberg, B.
Auteure/Auteur
Naud, M.C.
Auteure/Auteur
Jonet, L.
Auteure/Auteur
Besson-Lescure, B.
Auteure/Auteur
Jaisser, F.
Auteure/Auteur
Farman, N.
Auteure/Auteur
De Kozak, Y.
Auteure/Auteur
Behar-Cohen, F.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1932-6203
Statut éditorial
Publié
Date de publication
2012
Volume
7
Numéro
11
Première page
e49036
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
We have previously shown that the eye is a mineralocorticoid-sensitive organ and we now question the role of mineralocorticoid receptor (MR) in ocular inflammation. The endotoxin-induced uveitis (EIU), a rat model of human intraocular inflammation, was induced by systemic administration of lipopolysaccharide (LPS). Evaluations were made 6 and 24 hours after intraocular injection of aldosterone (simultaneous to LPS injection). Three hours after onset of EIU, the MR and the glucocorticoid metabolizing enzyme 11-beta hydroxysteroid dehydrogenase type 2 (11β-HSD2) expression were down-regulated in iris/ciliary body and the corticosterone concentration was increased in aqueous humor, altering the normal MR/glucocorticoid receptor (GR) balance. At 24 hours, the GR expression was also decreased. In EIU, aldosterone reduced the intensity of clinical inflammation in a dose-dependent manner. The clinical benefit of aldosterone was abrogated in the presence of the MR antagonist (RU26752) and only partially with the GR antagonist (RU38486). Aldosterone reduced the release of inflammatory mediators (6 and 24 hours: TNF-α, IFN-γ, MIP-1α) in aqueous humor and the number of activated microglia/macrophages. Aldosterone partly prevented the uveitis-induced MR down-regulation. These results suggest that MR expression and activation in iris/ciliary body could protect the ocular structures against damages induced by EIU.
Sujets
PID Serval
serval:BIB_7540DC6BC819
PMID
Open Access
Oui
Date de création
2013-08-19T14:03:46.823Z
Date de création dans IRIS
2025-05-21T00:32:02Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
23152847_BIB_7540DC6BC819.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
746.14 KB
Format
Adobe PDF
PID Serval
serval:BIB_7540DC6BC819.P001
URN
urn:nbn:ch:serval-BIB_7540DC6BC8198
Somme de contrôle
(MD5):e9b6cec43526299c357dfce8297c0d3e