Titre
Influence of statins on distinct circulating microRNAs during prolonged aerobic exercise.
Type
article
Institution
Externe
Périodique
Auteur(s)
Min, P.K.
Auteure/Auteur
Park, J.
Auteure/Auteur
Isaacs, S.
Auteure/Auteur
Taylor, B.A.
Auteure/Auteur
Thompson, P.D.
Auteure/Auteur
Troyanos, C.
Auteure/Auteur
D'Hemecourt, P.
Auteure/Auteur
Dyer, S.
Auteure/Auteur
Chan, S.Y.
Auteure/Auteur
Baggish, A.L.
Auteure/Auteur
Liens vers les personnes
ISSN
1522-1601
Statut éditorial
Publié
Date de publication
2016-03-15
Volume
120
Numéro
6
Première page
711
Dernière page/numéro d’article
720
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Statins exacerbate exercise-induced skeletal muscle injury. Muscle-specific microRNAs (myomiRs) increase in plasma after prolonged exercise, but the patterns of myomiRs release after statin-associated muscle injury have not been examined. We examined the relationships between statin exposure, in vitro and in vivo muscle contraction, and expression of candidate circulating myomiRs. We measured plasma levels of myomiRs, circulating microRNA-1 (c-miR-1), c-miR-133a, c-miR-206, and c-miR-499-5p from 28 statin-using and 28 nonstatin-using runners before (PRE), immediately after (FINISH), and 24 h after they ran a 42-km footrace (the 2011 Boston marathon) (POST-24). To examine these cellular-regulation myomiRs, we used contracting mouse C2C12 myotubes in culture with and without statin exposure to compare intracellular and extracellular expression of these molecules. In marathoners, c-miR-1, c-miR-133a, and c-miR-206 increased at FINISH, returned to baseline at POST-24, and were unaffected by statin use. In contrast, c-miR-499-5p was unchanged at FINISH but increased at POST-24 among statin users compared with PRE and runners who did not take statins. In cultured C2C12 myotubes, extracellular c-miR-1, c-miR-133a, and c-miR-206 were significantly increased by muscle contraction regardless of statin use. In contrast, extracellular miR-499-5p was unaffected by either isolated statin exposure or isolated carbachol exposure but it was increased when muscle contraction was combined with statin exposure. In summary, we found that statin-potentiated muscle injury during exercise is accompanied by augmented extracellular release of miR-499-5p. Thus c-miR-499-5p may serve as a biomarker of statin-potentiated muscle damage.
Sujets
PID Serval
serval:BIB_CB96A997BDAF
PMID
Date de création
2022-12-07T10:03:04.383Z
Date de création dans IRIS
2025-05-21T00:54:02Z