Titre
Fibrinmatrix erhöht Adhärenz von regenerativen peripheren Nervenzellen [Fibrin matrix enhances adherence of peripheral nerve regenerative cells]
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Kalbermatten, D.F.
Auteure/Auteur
Kingham, P.J.
Auteure/Auteur
Mahay, D.
Auteure/Auteur
Balcin, H.
Auteure/Auteur
Pierer, G.
Auteure/Auteur
Terenghi, G.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0722-1819
Statut éditorial
Publié
Date de publication
2008
Volume
40
Numéro
2
Première page
75
Dernière page/numéro d’article
80
Peer-reviewed
Oui
Langue
allemand
Résumé
Optimal seeding of a nerve conduit with cells is a core problem in tissue engineering of constructing an artificial nerve substitute to gap lesions in the peripheral nerve system. An ideal nerve gap substitute would have to present an equally distributed number of cells that can activate the regrowing axons. This work shows a new in vitro technique of two-step seeding of cells inside a conduit and on layered mats that allows a valuable targeting of the cells and a proven survival in the environment of poly-3-hydroxybutyrate (PHB) conduits. The technique uses two components of diluted fibrin glue Tisseel. Initially, the chosen area on the mat was coated with thrombin followed from the seeding of a fibrinogen-cell compound. Using Sprague Dawley rat cells, we could demonstrate with immunohistochemistry (S100, DAPI) techniques that undifferentiated (uMSC) and Schwann cells (SC) mimicking differentiated mesenchymal stem cells (dMSC) as well as SC can be suspended and targeted significantly better in dissolvable diluted fibrin glue than in growth medium. Analysis showed significantly better values for adherence (p < 0.001) and drop off (p < 0.05) from seeded cells. Using this two-step application allows the seeding of the cells to be more precise and simplifies the handling of cell transplantation.
PID Serval
serval:BIB_D3398D99E6D0
PMID
Date de création
2009-03-13T16:08:44.123Z
Date de création dans IRIS
2025-05-21T01:26:21Z