Titre
The trimeric G protein Go inflicts a double impact on axin in the Wnt/frizzled signaling pathway.
Type
article
Institution
Externe
Périodique
Auteur(s)
Egger-Adam, D.
Auteure/Auteur
Katanaev, V.L.
Auteure/Auteur
Liens vers les personnes
ISSN
1097-0177
Statut éditorial
Publié
Date de publication
2010
Volume
239
Numéro
1
Première page
168
Dernière page/numéro d’article
183
Langue
anglais
Résumé
The Wnt/Frizzled signaling pathway plays crucial roles in animal development and is deregulated in many cases of carcinogenesis. We and others have previously demonstrated that Frizzled proteins initiating the intracellular signaling are typical G protein-coupled receptors and rely on the trimeric G protein Go for Wnt transduction in Drosophila. However, the mode of action of Go and its interplay with other transducers of the pathway such as Dishevelled and Axin remained unclear. Here we show that the alpha-subunit of Go directly acts on Axin, the multidomain protein playing a negative role in the Wnt signaling. G alpha o physically binds Axin and re-localizes it to the plasma membrane. Furthermore, G alpha o suppresses Axin's inhibitory action on the Wnt pathway in Drosophila wing development. The interaction of G alpha o with Axin critically depends on the RGS domain of the latter. Additionally, we show that the betagamma-component of Go can directly bind and recruit Dishevelled from cytoplasm to the plasma membrane, where activated Dishevelled can act on the DIX domain of Axin. Thus, the two components of the trimeric Go protein mediate a double-direct and indirect-impact on different regions of Axin, which likely serves to ensure a robust inhibition of this protein and transduction of the Wnt signal.
Sujets
PID Serval
serval:BIB_AC1E648240D6
PMID
Date de création
2011-12-22T15:12:55.443Z
Date de création dans IRIS
2025-05-21T01:51:41Z
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Nom
BIB_AC1E648240D6.P001.pdf
Version du manuscrit
published
Taille
7.75 MB
Format
Adobe PDF
PID Serval
serval:BIB_AC1E648240D6.P001
Somme de contrôle
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