Titre
Elastic membrane heterogeneity of living cells revealed by stiff nanoscale membrane domains.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Roduit, C.
Auteure/Auteur
van der Goot, F.G.
Auteure/Auteur
De Los Rios, P.
Auteure/Auteur
Yersin, A.
Auteure/Auteur
Steiner, P.
Auteure/Auteur
Dietler, G.
Auteure/Auteur
Catsicas, S.
Auteure/Auteur
Lafont, F.
Auteure/Auteur
Kasas, S.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1542-0086
Statut éditorial
Publié
Date de publication
2008
Volume
94
Numéro
4
Première page
1521
Dernière page/numéro d’article
1532
Peer-reviewed
Oui
Langue
anglais
Notes
Journal article --- Old month value: Nov 2
Résumé
Many approaches have been developed to characterize the heterogeneity of membranes in living cells. In this study, the elastic properties of specific membrane domains in living cells are characterized by atomic force microscopy. Our data reveal the existence of heterogeneous nanometric scale domains with specific biophysical properties. We focused on glycosylphosphatidylinositol (GPI)-anchored proteins, which play an important role in membrane trafficking and cell signaling under both physiological and pathological conditions and which are known to partition preferentially into cholesterol-rich microdomains. We demonstrate that these GPI-anchored proteins reside within domains that are stiffer than the surrounding membrane. In contrast, membrane domains containing the transferrin receptor, which does not associate with cholesterol-rich regions, manifest no such feature. The heightened stiffness of GPI domains is consistent with existing data relating to the specific condensation of lipids and the slow diffusion rates of lipids and proteins therein. Our quantitative data may forge the way to unveiling the links that exist between membrane stiffness, molecular diffusion, and signaling activation.
PID Serval
serval:BIB_C56D635DC857
PMID
Open Access
Oui
Date de création
2008-01-24T13:24:26.682Z
Date de création dans IRIS
2025-05-21T02:25:20Z