Titre
A New Tool for In Vivo Study of Astrocyte Connexin 43 in Brain.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Droguerre, M.
Auteure/Auteur
Tsurugizawa, T.
Auteure/Auteur
Duchêne, A.
Auteure/Auteur
Portal, B.
Auteure/Auteur
Guiard, B.P.
Auteure/Auteur
Déglon, N.
Auteure/Auteur
Rouach, N.
Auteure/Auteur
Hamon, M.
Auteure/Auteur
Mouthon, F.
Auteure/Auteur
Ciobanu, L.
Auteure/Auteur
Charvériat, M.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2045-2322
Statut éditorial
Publié
Date de publication
2019-12-04
Volume
9
Numéro
1
Première page
18292
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
Astrocytes are glial cells organized in dynamic and structured networks in the brain. These plastic networks, involving key proteins such as connexin 43 (Cx43), are engaged in fine neuronal tuning and have recently been considered as emerging therapeutic targets in central nervous system disorders. We developed and validated a new application of the manganese-enhanced magnetic resonance imaging (MEMRI) technique allowing in vivo investigations of astrocyte-neuron interactions through quantification of brain Cx43 functional activity. The proof of concept has been achieved by quantification of MEMRI signals in brain after either local astrocyte-specific Cx43 knockdown with shRNA or systemic administration of Cx43 blockers. Unilateral hippocampal Cx43 genetical silencing was associated with an ipsilateral local increase of MEMRI signal. Furthermore, Cx43 blockers also enhanced MEMRI signal responses in hippocampus. Altogether, these data reveal the MEMRI technique as a tool for quantitative imaging of in vivo Cx43-dependent function in astrocytes under physiological and pathological conditions.
Sujets
PID Serval
serval:BIB_7C1615ED7809
PMID
Open Access
Oui
Date de création
2019-12-11T12:09:19.728Z
Date de création dans IRIS
2025-05-21T02:34:46Z
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Nom
Droguerre-2019-A New Tool for In Vivo Study of.pdf
Version du manuscrit
preprint
Licence
https://creativecommons.org/licenses/by/4.0
Taille
4.35 MB
Format
Adobe PDF
PID Serval
serval:BIB_7C1615ED7809.P001
URN
urn:nbn:ch:serval-BIB_7C1615ED78094
Somme de contrôle
(MD5):3347b9ba4bed4caf6f88dbae747156ad