Titre
Regional variations in ABC transporter expression along the mouse intestinal tract.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Mutch, D.M.
Auteure/Auteur
Anderle, P.
Auteure/Auteur
Fiaux, M.
Auteure/Auteur
Mansourian, R.
Auteure/Auteur
Vidal, K.
Auteure/Auteur
Wahli, W.
Auteure/Auteur
Williamson, G.
Auteure/Auteur
Roberts, M.A.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1531-2267
Statut éditorial
Publié
Date de publication
2004
Volume
17
Numéro
1
Première page
11
Dernière page/numéro d’article
20
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Multicenter Study ; Validation Studies
Publication Status: epublish
Publication Status: epublish
Résumé
The ATP-binding cassette (ABC) family of proteins comprise a group of membrane transporters involved in the transport of a wide variety of compounds, such as xenobiotics, vitamins, lipids, amino acids, and carbohydrates. Determining their regional expression patterns along the intestinal tract will further characterize their transport functions in the gut. The mRNA expression levels of murine ABC transporters in the duodenum, jejunum, ileum, and colon were examined using the Affymetrix MuU74v2 GeneChip set. Eight ABC transporters (Abcb2, Abcb3, Abcb9, Abcc3, Abcc6, Abcd1, Abcg5, and Abcg8) displayed significant differential gene expression along the intestinal tract, as determined by two statistical models (a global error assessment model and a classic ANOVA, both with a P < 0.01). Concordance with semiquantitative real-time PCR was high. Analyzing the promoters of the differentially expressed ABC transporters did not identify common transcriptional motifs between family members or with other genes; however, the expression profile for Abcb9 was highly correlated with fibulin-1, and both genes share a common complex promoter model involving the NFkappaB, zinc binding protein factor (ZBPF), GC-box factors SP1/GC (SP1F), and early growth response factor (EGRF) transcription binding motifs. The cellular location of another of the differentially expressed ABC transporters, Abcc3, was examined by immunohistochemistry. Staining revealed that the protein is consistently expressed in the basolateral compartment of enterocytes along the anterior-posterior axis of the intestine. Furthermore, the intensity of the staining pattern is concordant with the expression profile. This agrees with previous findings in which the mRNA, protein, and transport function of Abcc3 were increased in the rat distal intestine. These data reveal regional differences in gene expression profiles along the intestinal tract and demonstrate that a complete understanding of intestinal ABC transporter function can only be achieved by examining the physiologically distinct regions of the gut.
Sujets
PID Serval
serval:BIB_7CBC6E5E85AA
PMID
Date de création
2008-01-24T15:04:50.503Z
Date de création dans IRIS
2025-05-21T03:45:10Z