Titre
Atomic force microscopy of nucleoprotein complexes.
Type
synthèse (review)
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Scanning Microscopy
Auteur(s)
Lyubchenko, Y.L.
Auteure/Auteur
Jacobs, B.L.
Auteure/Auteur
Lindsay, S.M.
Auteure/Auteur
Stasiak, A.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0891-7035[print], 0891-7035[linking]
Statut éditorial
Publié
Date de publication
1995-09
Volume
9
Numéro
3
Première page
705
Dernière page/numéro d’article
24; discussion 724-7
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S. ; Review
Publication Status: ppublish
Publication Status: ppublish
Résumé
Recent data on the AFM studies of nucleoprotein complexes of different types are reviewed in this paper. The first section describes the progress in the sample preparation methods for AFM studies of nucleic acids and nucleoprotein complexes. The second part of this paper reviews AFM data on studies of complexes of DNA with regulatory proteins. These studies include two different types of DNA distortion induced by proteins binding: local bending of DNA at sites of protein binding and formation of large loops due to protein-protein interactions between molecules bound to distant sites along the DNA molecules (DNA looping). The prospects for use of AFM for physical mapping of genomes are discussed in this section as well. The third part of the paper reviews data on studies of complexes of DNA with non-sequence specific binding proteins. Special emphasis is given to studies of chromatin which have resulted in progress in the understanding of structure of native chromatin fiber. In this section, novel data on AFM studies of RecA-DNA filaments and complexes of dsRNA with the dsRNA-specific protein p25 are also presented. Discussion of the substrate preparation procedures in relation to the AFM studies of nucleoprotein complexes is given in the final section.
PID Serval
serval:BIB_BC7EDE6F728D
PMID
Date de création
2008-01-24T09:36:14.371Z
Date de création dans IRIS
2025-05-21T03:53:04Z