Titre
Photorepair of ultraviolet radiation-induced pyrimidine dimers in corneal DNA
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Ley, R. D.
Auteure/Auteur
Applegate, L. A.
Auteure/Auteur
Freeman, S. E.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0027-5107
Statut éditorial
Publié
Date de publication
1988
Volume
194
Numéro
1
Première page
49
Dernière page/numéro d’article
55
Notes
DA - 19880804
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Pyrimidine Dimers)
SB - IM
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Pyrimidine Dimers)
SB - IM
Résumé
The induction and photorepair of pyrimidine dimers in DNA have been measured in the ultraviolet-irradiated, corneal epithelium of the marsupial, Monodelphis domestica, using damage-specific nucleases from Micrococcus luteus in conjunction with agarose gel electrophoresis. We observed that FS-40 sunlamps (280-400 nm) induced 7.2 +/- 1.0 X 10(-5) pyrimidine dimers per kilobase (kb) of DNA per J/m2. Following 100 J/m2, 50% and greater than 90% of the dimers were photorepaired during a 10- and 30-min exposure to photoreactivating light (320-400 nm), respectively. In addition, approximately 70% and approximately 60% of the dimers induced by 300 and 500 J/m2, respectively, were repaired by a 60-min exposure to photoreactivating light. The capacity of the corneal epithelium of M. domestica to photorepair pyrimidine dimers identifies this animal as a potentially useful model with which to determine whether pyrimidine dimers are involved in pathological changes of the irradiated eye
Sujets
PID Serval
serval:BIB_CD97E44F02D5
PMID
Date de création
2008-02-18T16:33:11.508Z
Date de création dans IRIS
2025-05-21T03:57:12Z