Titre
Genes and loops in 320,000 base-pairs of the Drosophila melanogaster chromosome
Type
article
Institution
Externe
Périodique
Auteur(s)
Mirkovitch, J.
Auteure/Auteur
Spierer, P.
Auteure/Auteur
Laemmli, U. K.
Auteure/Auteur
Liens vers les personnes
ISSN
0022-2836
Statut éditorial
Publié
Date de publication
1986-07
Volume
190
Numéro
2
Première page
255
Dernière page/numéro d’article
8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul 20
Research Support, Non-U.S. Gov't --- Old month value: Jul 20
Résumé
We have mapped the DNA sequences bound to the nuclear scaffold along 320,000 base-pairs of a genetically well-defined region of the Drosophila chromosome. We have found that the domains delimited by the scaffold attachment regions are heterogeneous in size (ranging from 26,000 to 112,000 base-pairs in this interval), and that the attachment sites are within unique sequences as judged by blot hybridization. We also found that looped domains contain up to five, or even eight, unrelated genes including, in some cases, more than one transcribed gene. The loop organization unravelled here in cultured cells does not correspond to the banding pattern seen in salivary gland polytene chromosomes.
Sujets
PID Serval
serval:BIB_8AD0CC74260F
PMID
Date de création
2008-01-25T07:41:53.038Z
Date de création dans IRIS
2025-05-21T04:13:54Z