Titre
Toward synthetic combinatorial peptide libraries in positional scanning format (PS-SCL)-based identification of CD8+ Tumor-reactive T-Cell Ligands: a comparative analysis of PS-SCL recognition by a single tumor-reactive CD8+ cytolytic T-lymphocyte clone.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Rubio-Godoy, V.
Auteure/Auteur
Pinilla, C.
Auteure/Auteur
Dutoit, V.
Auteure/Auteur
Borras, E.
Auteure/Auteur
Simon, R.
Auteure/Auteur
Zhao, Y.
Auteure/Auteur
Cerottini, J.C.
Auteure/Auteur
Romero, P.
Auteure/Auteur
Houghten, R.
Auteure/Auteur
Valmori, D.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0008-5472
Statut éditorial
Publié
Date de publication
2002
Volume
62
Numéro
7
Première page
2058
Dernière page/numéro d’article
2063
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
The use of synthetic combinatorial peptide libraries in positional scanning format (PS-SCL) has emerged recently as an alternative approach for the identification of peptides recognized by T lymphocytes. The choice of both the PS-SCL used for screening experiments and the method used for data analysis are crucial for implementing this approach. With this aim, we tested the recognition of different PS-SCL by a tyrosinase 368-376-specific CTL clone and analyzed the data obtained with a recently developed biometric data analysis based on a model of independent and additive contribution of individual amino acids to peptide antigen recognition. Mixtures defined with amino acids present at the corresponding positions in the native sequence were among the most active for all of the libraries. Somewhat surprisingly, a higher number of native amino acids were identifiable by using amidated COOH-terminal rather than free COOH-terminal PS-SCL. Also, our data clearly indicate that when using PS-SCL longer than optimal, frame shifts occur frequently and should be taken into account. Biometric analysis of the data obtained with the amidated COOH-terminal nonapeptide library allowed the identification of the native ligand as the sequence with the highest score in a public human protein database. However, the adequacy of the PS-SCL data for the identification for the peptide ligand varied depending on the PS-SCL used. Altogether these results provide insight into the potential of PS-SCL for the identification of CTL-defined tumor-derived antigenic sequences and may significantly implement our ability to interpret the results of these analyses.
PID Serval
serval:BIB_A885131022B3
PMID
Date de création
2008-01-28T10:13:31.604Z
Date de création dans IRIS
2025-05-21T04:18:36Z