Titre
HdaB: a novel and conserved DnaA-related protein that targets the RIDA process to stimulate replication initiation.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Frandi, A.
Auteure/Auteur
Collier, J.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1362-4962
Statut éditorial
Publié
Date de publication
2020-03-18
Volume
48
Numéro
5
Première page
2412
Dernière page/numéro d’article
2423
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Exquisite control of the DnaA initiator is critical to ensure that bacteria initiate chromosome replication in a cell cycle-coordinated manner. In many bacteria, the DnaA-related and replisome-associated Hda/HdaA protein interacts with DnaA to trigger the Regulatory Inactivation of DnaA (RIDA) and prevent over-initiation events. In the Caulobacter crescentus Alphaproteobacterium, the RIDA process also targets DnaA for its rapid proteolysis by Lon. The impact of the RIDA process on adaptation of bacteria to changing environments remains unexplored. Here, we identify a novel and conserved DnaA-related protein, named HdaB, and show that homologs from three different Alphaproteobacteria can inhibit the RIDA process, leading to over-initiation and cell death when expressed in actively growing C. crescentus cells. We further show that HdaB interacts with HdaA in vivo, most likely titrating HdaA away from DnaA. Strikingly, we find that HdaB accumulates mainly during stationary phase and that it shortens the lag phase upon exit from stationary phase. Altogether, these findings suggest that expression of hdaB during stationary phase prepares cells to restart the replication of their chromosome as soon as conditions improve, a situation often met by free-living or facultative intracellular Alphaproteobacteria.
PID Serval
serval:BIB_72742351BADF
PMID
Open Access
Oui
Date de création
2020-01-03T12:01:24.377Z
Date de création dans IRIS
2025-05-21T04:34:40Z
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Nom
31875223_BIB_72742351BADF.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by-nc/4.0
Taille
5.65 MB
Format
Adobe PDF
PID Serval
serval:BIB_72742351BADF.P001
URN
urn:nbn:ch:serval-BIB_72742351BADF4
Somme de contrôle
(MD5):1268a3ed59aec7de1995bf14993524ba