Titre

Investigation of the global protein content from healthy human tears.

Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Dor, M.
Auteure/Auteur
Eperon, S.
Auteure/Auteur
Lalive, P.H.
Auteure/Auteur
Guex-Crosier, Y.
Auteure/Auteur
Hamedani, M.
Auteure/Auteur
Salvisberg, C.
Auteure/Auteur
Turck, N.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1096-0007
Statut éditorial
Publié
Date de publication
2019-02
Volume
179
Première page
64
Dernière page/numéro d’article
74
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Considering absence of invasiveness and side effects, tears emerge as a particularly attractive fluid for biomarker discovery and therefore for daily clinical use. However, to date this fluid remains poorly studied in healthy condition. Here, we present an updated in-depth characterisation of the human healthy tear protein composition using proteomics approach. Both eyes of 8 healthy controls (4 men and 4 women, average age: 38 ± 18) were collected using the Schirmer's strip method. After liquid digestion and off-gel electrophoresis fractionation, three independent proteomics analyses were performed on a LTQ-Orbitrap Velos Pro. Globally, 1351 proteins were identified with 2 unique peptides and 1% FDR. Gene ontology analyses showed up that 39% of the tear proteins were enzymes, with high numbers of dehydrogenases, phosphatases, kinases and ligases. Immunoglobulins, serpins and 14-3-3 domains proteins also emerged as the main tear protein families. The glycolysis and the coagulation and complement cascades, which were already shown in tears as involved in ocular and systemic diseases, were highlighted performing pathway analyses. Our study therefore complements the existing data on healthy tears proteome. Nevertheless, extensive studies for deeply and definitively characterise this promising fluid are required in the near future in order to be able to routinely use this fluid in clinics. A better understanding of its protein content will probably open new avenues in the biomarker discovery and clinical practice in the near future.
Sujets

Adult

Biomarkers/metabolism...

Electrophoresis, Poly...

Eye Proteins/metaboli...

Female

Healthy Volunteers

Humans

Male

Mass Spectrometry

Middle Aged

Proteomics

Tears/metabolism

Young Adult

Gas-phase fractionati...

Integrative pathways

Off-gel electrophores...

Proteome

Tears

PID Serval
serval:BIB_74008C08D4B0
DOI
PMID
WOS
Permalien
Date de création
2018-10-30T11:38:31.894Z
Date de création dans IRIS
2025-05-21T04:35:46Z
Fichier(s)
En cours de chargement...
Vignette d'image
Nom

30321513_PréPrint.pdf

Version du manuscrit

preprint

Taille

1.2 MB

Format

Adobe PDF

PID Serval

serval:BIB_74008C08D4B0.P001

URN

urn:nbn:ch:serval-BIB_74008C08D4B07

Somme de contrôle

(MD5):dfc3fee02acef6bb0c5222e2a8096ac2

En cours de chargement...
Vignette d'image
Nom

Tear proteome S1 supp file.pdf

Version du manuscrit

supplementary

Taille

12.83 MB

Format

Adobe PDF

PID Serval

serval:BIB_74008C08D4B0.S001

Somme de contrôle

(MD5):323f22a86753fc029aa2dffdf401cdee

En cours de chargement...
Vignette d'image
Nom

Tear proteome S2 supp file.pdf

Version du manuscrit

supplementary

Taille

207.18 KB

Format

Adobe PDF

PID Serval

serval:BIB_74008C08D4B0.S002

Somme de contrôle

(MD5):a9a3ebd3cef18dcd2fc7249ca1e24866

En cours de chargement...
Vignette d'image
Nom

Tear proteome S3 supp file.pdf

Version du manuscrit

supplementary

Taille

85.05 KB

Format

Adobe PDF

PID Serval

serval:BIB_74008C08D4B0.S003

Somme de contrôle

(MD5):0758b83ba687295779b9037844c526dd

En cours de chargement...
Vignette d'image
Nom

Tear proteome S4 supp file.pdf

Version du manuscrit

supplementary

Taille

199.17 KB

Format

Adobe PDF

PID Serval

serval:BIB_74008C08D4B0.S004

Somme de contrôle

(MD5):916a9aab98142e6585b0f71af2e32398