Titre
Rearrangements of minisatellites in the human telomerase reverse transcriptase gene are not correlated with its expression in colon carcinomas
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Szutorisz, H.
Auteure/Auteur
Palmqvist, R.
Auteure/Auteur
Roos, G.
Auteure/Auteur
Stenling, R.
Auteure/Auteur
Schorderet, D. F.
Auteure/Auteur
Reddel, R.
Auteure/Auteur
Lingner, J.
Auteure/Auteur
Nabholz, M.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0950-9232
Statut éditorial
Publié
Date de publication
2001-05
Volume
20
Numéro
20
Première page
2600
Dernière page/numéro d’article
5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 3
Research Support, Non-U.S. Gov't --- Old month value: May 3
Résumé
Telomerase activation is crucial in human carcinogenesis. The limiting component of telomerase, the catalytic subunit (hTERT), is undetectable in normal somatic cells but present in most tumor cells, including the earliest stages of colon carcinoma. The mechanisms involved in the differential expression in normal and tumor cells are not understood. In normal cells hTERT expression is shut down by a repressor, and upregulation could be a consequence of cis-acting changes in the hTERT gene, making it resistant to repression. We have identified a polymorphic and a monomorphic minisatellite in the second intron of the hTERT gene, and polymorphic one in intron 6. The polymorphic minisatellite in intron 2 contains binding sites for c-Myc, which has been shown to upregulate hTERT transcription. Screening colon carcinoma DNAs for rearrangements of hTERT minisatellites we detected no changes in 33 samples from tumors, most of which express hTERT. This indicates that size rearrangements of the hTERT minisatellites are not required for telomerase expression in colon carcinomas. Minor changes and one LOH were seen in five tumors.
Sujets
PID Serval
serval:BIB_918226DFA05B
PMID
Open Access
Oui
Date de création
2008-01-28T11:59:01.195Z
Date de création dans IRIS
2025-05-21T04:50:54Z