Titre

MarrowCellDLD: a microfluidic method for label-free retrieval of fragile bone marrow-derived cells.

Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Porro, G.
Co-première auteure/Co-premier auteur
Sarkis, R.
Co-première auteure/Co-premier auteur
Obergozo, C.
Auteure/Auteur
Godot, L.
Auteure/Auteur
Amato, F.
Auteure/Auteur
Humbert, M.
Auteure/Auteur
Naveiras, O.
Co-dernière auteure/Co-dernier auteur
Guiducci, C.
Co-dernière auteure/Co-dernier auteur
Liens vers les personnes
Liens vers les unités
ISSN
2045-2322
Statut éditorial
Publié
Date de publication
2023-12-18
Volume
13
Numéro
1
Première page
22462
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Functional bone marrow studies have focused primarily on hematopoietic progenitors, leaving limited knowledge about other fragile populations, such as bone marrow adipocytes (BMAds) and megakaryocytes. The isolation of these cells is challenging due to rupture susceptibility and large size. We introduce here a label-free cytometry microsystem, MarrowCellDLD, based on deterministic lateral displacement. MarrowCellDLD enables the isolation of large, fragile BM-derived cells based on intrinsic size properties while preserving their viability and functionality. Bone marrow adipocytes, obtained from mouse and human stromal line differentiation, as well as megakaryocytes, from primary human CD34+ hematopoietic stem and progenitor cells, were used for validation. Precise micrometer-range separation cutoffs were adapted for each cell type. Cells were sorted directly in culture media, without pre-labeling steps, and with real-time imaging for quality control. At least 10 <sup>6</sup> cells were retrieved intact per sorting round. Our method outperformed two FACS instruments in purity and yield, particularly for large cell size fractions. MarrowCellDLD represents a non-destructive sorting tool for large, fragile BM-derived cells, facilitating the separation of pure populations of BMAds and megakaryocytes to further investigate their physiological and pathological roles.
Sujets

Humans

Mice

Animals

Hematopoietic Stem Ce...

Bone Marrow

Microfluidics

Cell Separation/metho...

Megakaryocytes

Antigens, CD34

Bone Marrow Cells

Cell Differentiation

Flow Cytometry

PID Serval
serval:BIB_EE628D166E3F
DOI
PMID
Permalien
Open Access
Oui
Date de création
2023-12-21T15:18:17.885Z
Date de création dans IRIS
2025-05-21T05:19:16Z
Fichier(s)
En cours de chargement...
Vignette d'image
Nom

s41598-023-47978-w (1).pdf

Version du manuscrit

published

Licence

https://creativecommons.org/licenses/by/4.0

Taille

2.69 MB

Format

Adobe PDF

PID Serval

serval:BIB_EE628D166E3F.P001

URN

urn:nbn:ch:serval-BIB_EE628D166E3F8

Somme de contrôle

(MD5):a1c63ff457a8284a06ece9d4d9e4014a

En cours de chargement...
Vignette d'image
Nom

41598_2023_47978_MOESM1_ESM.docx

Version du manuscrit

supplementary

Taille

2.23 MB

Format

Microsoft Word XML

PID Serval

serval:BIB_EE628D166E3F.S001

Somme de contrôle

(MD5):e054c928fa3d73ddc8b792fecea3b449