Titre
Recovery of a persistent Canine distemper virus expressing the enhanced green fluorescent protein from cloned cDNA.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Plattet, P.
Auteure/Auteur
Zweifel, C.
Auteure/Auteur
Wiederkehr, C.
Auteure/Auteur
Belloy, L.
Auteure/Auteur
Cherpillod, P.
Auteure/Auteur
Zurbriggen, A.
Auteure/Auteur
Wittek, R.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0168-1702[print], 0168-1702[linking]
Statut éditorial
Publié
Date de publication
2004-05
Volume
101
Numéro
2
Première page
147
Dernière page/numéro d’article
153
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Wild-type A75/17-Canine distemper virus (CDV) is a highly virulent strain, which induces a persistent infection in the central nervous system (CNS) with demyelinating disease. Wild-type A75/17-CDV, which is unable to replicate in cell lines to detectable levels, was adapted to grow in Vero cells and was designated A75/17-V. Sequence comparison between the two genomes revealed seven nucleotide differences located in the phosphoprotein (P), the matrix (M) and the large (L) genes. The P gene is polycistronic and encodes two auxiliary proteins, V and C, besides the P protein. The mutations resulted in amino acid changes in the P and V, but not in the C protein, as well as in the M and L proteins. Here, a rescue system was developed for the A75/17-V strain, which was shown to be attenuated in vivo, but retains a persistent infection phenotype in Vero cells. In order to track the recombinant virus, an additional transcription unit coding for the enhanced green fluorescent protein (eGFP) was inserted at the 3' proximal position in the A75/17-V cDNA clone. Reverse genetics technology will allow us to characterize the genetic determinants of A75/17-V CDV persistent infection in cell culture.
Sujets
PID Serval
serval:BIB_E09120ADE622
PMID
Date de création
2008-01-24T09:43:37.990Z
Date de création dans IRIS
2025-05-21T05:27:11Z