Titre
Neuropathic Pain Phenotype Does Not Involve the NLRP3 Inflammasome and Its End Product Interleukin-1β in the Mice Spared Nerve Injury Model.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Curto-Reyes, V.
Auteure/Auteur
Kirschmann, G.
Auteure/Auteur
Pertin, M.
Auteure/Auteur
Drexler, S.K.
Auteure/Auteur
Decosterd, I.
Auteure/Auteur
Suter, M.R.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1932-6203
Statut éditorial
Publié
Date de publication
2015
Volume
10
Numéro
7
Première page
e0133707
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: epublish
Résumé
The NACHT, LRR and PYD domains-containing protein 3 (NLRP3) inflammasome is one of the main sources of interleukin-1β (IL-1β) and is involved in several inflammatory-related pathologies. To date, its relationship with pain has not been studied in depth. The aim of our study was to elucidate the role of NLRP3 inflammasome and IL-1β production on neuropathic pain. Results showed that basal pain sensitivity is unaltered in NLRP3-/- mice as well as responses to formalin test. Spared nerve injury (SNI) surgery induced the development of mechanical allodynia and thermal hyperalgesia in a similar way in both genotypes and did not modify mRNA levels of the NLRP3 inflammasome components in the spinal cord. Intrathecal lipopolysaccharide (LPS) injection increases apoptosis-associated speck like protein (ASC), caspase-1 and IL-1β expression in both wildtype and NLRP3-/- mice. Those data suggest that NLRP3 is not involved in neuropathic pain and also that other sources of IL-1β are implicated in neuroinflammatory responses induced by LPS.
Sujets
PID Serval
serval:BIB_DEBB7BE677C8
PMID
Open Access
Oui
Date de création
2015-07-29T08:46:29.522Z
Date de création dans IRIS
2025-05-21T05:57:20Z
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Nom
BIB_DEBB7BE677C8.P001.pdf
Version du manuscrit
preprint
Taille
787.52 KB
Format
Adobe PDF
PID Serval
serval:BIB_DEBB7BE677C8.P001
URN
urn:nbn:ch:serval-BIB_DEBB7BE677C80
Somme de contrôle
(MD5):feed897acfae841985264e47a4d335be