Titre
Spectrally resolved fluorescence correlation spectroscopy based on global analysis.
Type
article
Institution
Externe
Périodique
Auteur(s)
Previte, M.J.
Auteure/Auteur
Pelet, S.
Auteure/Auteur
Kim, K.H.
Auteure/Auteur
Buehler, C.
Auteure/Auteur
So, P.T.
Auteure/Auteur
Liens vers les personnes
ISSN
1520-6882
Statut éditorial
Publié
Date de publication
2008
Volume
80
Numéro
9
Première page
3277
Dernière page/numéro d’article
3284
Langue
anglais
Résumé
Multicolor fluorescence correlation spectroscopy has been recently developed to study chemical interactions of multiple chemical species labeled with spectrally distinct fluorophores. In the presence of spectral overlap, there exists a lower detectability limit for reaction products with multicolor fluorophores. In addition, the ability to separate bound product from reactants allows thermodynamic properties such as dissociation constants to be measured for chemical reactions. In this report, we utilize a spectrally resolved two-photon microscope with single-photon counting sensitivity to acquire spectral and temporal information from multiple chemical species. Further, we have developed a global fitting analysis algorithm that simultaneously analyzes all distinct auto- and cross-correlation functions from 15 independent spectral channels. We have demonstrated that the global analysis approach allows the concentration and diffusion coefficients of fluorescent particles to be resolved despite the presence of overlapping emission spectra.
PID Serval
serval:BIB_E007658FCB54
PMID
Date de création
2012-09-25T13:42:34.707Z
Date de création dans IRIS
2025-05-21T06:08:24Z