Titre
Transcriptome of a mouse kidney cortical collecting duct cell line: effects of aldosterone and vasopressin.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Robert-Nicoud, M.
Auteure/Auteur
Flahaut, M.
Auteure/Auteur
Elalouf, J.M.
Auteure/Auteur
Nicod, M.
Auteure/Auteur
Salinas, M.
Auteure/Auteur
Bens, M.
Auteure/Auteur
Doucet, A.
Auteure/Auteur
Wincker, P.
Auteure/Auteur
Artiguenave, F.
Auteure/Auteur
Horisberger, J.D.
Auteure/Auteur
Vandewalle, A.
Auteure/Auteur
Rossier, B.C.
Auteure/Auteur
Firsov, D.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0027-8424
Statut éditorial
Publié
Date de publication
2001
Volume
98
Numéro
5
Première page
2712
Dernière page/numéro d’article
2716
Langue
anglais
Résumé
Aldosterone and vasopressin are responsible for the final adjustment of sodium and water reabsorption in the kidney. In principal cells of the kidney cortical collecting duct (CCD), the integral response to aldosterone and the long-term functional effects of vasopressin depend on transcription. In this study, we analyzed the transcriptome of a highly differentiated mouse clonal CCD principal cell line (mpkCCD(cl4)) and the changes in the transcriptome induced by aldosterone and vasopressin. Serial analysis of gene expression (SAGE) was performed on untreated cells and on cells treated with either aldosterone or vasopressin for 4 h. The transcriptomes in these three experimental conditions were determined by sequencing 169,721 transcript tags from the corresponding SAGE libraries. Limiting the analysis to tags that occurred twice or more in the data set, 14,654 different transcripts were identified, 3,642 of which do not match known mouse sequences. Statistical comparison (at P < 0.05 level) of the three SAGE libraries revealed 34 AITs (aldosterone-induced transcripts), 29 ARTs (aldosterone-repressed transcripts), 48 VITs (vasopressin-induced transcripts) and 11 VRTs (vasopressin-repressed transcripts). A selection of the differentially-expressed, hormone-specific transcripts (5 VITs, 2 AITs and 1 ART) has been validated in the mpkCCD(cl4) cell line either by Northern blot hybridization or reverse transcription-PCR. The hepatocyte nuclear transcription factor HNF-3-alpha (VIT39), the receptor activity modifying protein RAMP3 (VIT48), and the glucocorticoid-induced leucine zipper protein (GILZ) (AIT28) are candidate proteins playing a role in physiological responses of this cell line to vasopressin and aldosterone.
PID Serval
serval:BIB_EAC34437024B
PMID
Open Access
Oui
Date de création
2008-01-24T11:32:20.723Z
Date de création dans IRIS
2025-05-21T06:21:20Z