Titre
Exposure to bioaerosols in poultry houses at different stages of fattening: use of real-time PCR for airborne bacterial quantification
Type
abstract de conférence/colloque
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Oppliger, Anne
Auteure/Auteur
Charrière, Nicole
Auteure/Auteur
Droz, Pierre-Olivier
Auteure/Auteur
Rinsoz, Thomas
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
Maison d’édition
Université de Lausanne, Faculté de biologie et de médecine
Titre du livre ou conférence/colloque
Regenerative medicine, CHUV Research Day, January 17, 2008
Adresse
Lausanne
Statut éditorial
Publié
Date de publication
2008
Première page
ENA
Dernière page/numéro d’article
1
Langue
anglais
Notes
Type de document : Abstracts SAPHIRID:67685
Résumé
Previous studies have demonstrated that poultry-house workers are exposed to very high levels of organic dust and consequently have an increased prevalence of adverse respiratory symptoms. However, the influence of the age of broilers, on bioaerosol concentrations has not been investigated. To evaluate the evolution of bioaerosol concentration during the fattening period, bioaerosol parameters (inhalable dust, endotoxin and bacteria) were measured in 12 poultry confinement buildings in Switzerland, at 3 different stages of the birds' growth; Samples of air taken from within the breathing zones of individual poultry-house employees as they caught the chickens ready to be transported for slaughter, were also analysed. Quantitative PCR (Q-PCR) was used to assess the quantity of total airborne bacteria and total airborne Staphylococcus species. Bioaerosol levels increased significantly during the fattening period of the chickens. During the task of catching mature birds, the mean inhalable dust concentration for a worker was 31 ± 4.7 mg/m3, and endotoxin concentration was 11'080 ± 3436 UE/m3 air, more than ten-fold higher than the Swiss occupational recommended value (1000 UE/m3). The mean exposure level of bird catchers to total bacteria and Staphylococcus species measured by Q-PCR is also very high, respectively reaching values of 72 (± 11) x107 cells/m3 air and 70 (± 16) x106/m3 air. It was concluded that in the absence of wearing protective breathing apparatus, chicken catchers in Switzerland risk exposure beyond recommended limits for all measured bioaerosol parameters. Moreover, the use of Q-PCR to estimate total and specific numbers of airborne bacteria is a promising tool for evaluating any modifications intended to improve the safety of current working practices.
PID Serval
serval:BIB_E80A6A0D1CA3
Date de création
2009-03-18T11:16:36.361Z
Date de création dans IRIS
2025-05-21T06:29:34Z
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Nom
BIB_E80A6A0D1CA3.P001.pdf
Version du manuscrit
preprint
Taille
322.84 KB
Format
Adobe PDF
PID Serval
serval:BIB_E80A6A0D1CA3.P001
URN
urn:nbn:ch:serval-BIB_E80A6A0D1CA37
Somme de contrôle
(MD5):e3097f523a112e779734f6d0f72a25c4