Titre
The CaMV 35S promoter has a weak expression activity in dark grown tissues of moss Physcomitrella patens
Type
article
Institution
Externe
Périodique
Plant Signal Behav
Auteur(s)
Saidi, Younousse
Auteure/Auteur
Schaefer, Didier G
Auteure/Auteur
Goloubinoff, Pierre
Auteure/Auteur
Zrÿd, Jean-Pierre
Auteure/Auteur
Finka, Andrija
Auteure/Auteur
Liens vers les personnes
Statut éditorial
Publié
Date de publication
2009-05
Volume
4
Numéro
5
Première page
457
Dernière page/numéro d’article
459
Peer-reviewed
Oui
Langue
anglais
Résumé
The constitutive Cauliflower Mosaic Virus 35S promoter (CaMV 35S) is widely used as a tool to express recombinant proteins in plants, but with different success. We previously showed that the expression of an F-actin marker, GFP-talin, in Physcomitrella patens using the CaMV 35S promoter failed to homogenously label moss tissues. Here, we show a significant diminution of the GFP fluorescence in dark grown old moss cells and complete lack of labelling in newly differentiated cells. Furthermore, we demonstrate that stable moss lines harbouring a resistance cassette driven by the CaMV 35S are unable to grow in darkness in the presence of the antibiotic. In contrast to the CaMV 35S, the heat inducible promoter, hsp17.3B showed uniform expression pattern in all cells and tissues following a mild heat shock.
Sujets
PID Serval
serval:BIB_F95A9A9239F2
Date de création
2011-01-06T11:06:17.796Z
Date de création dans IRIS
2025-05-21T06:29:47Z