Titre
Real-time, in vivo intracellular recordings of caterpillar-induced depolarization waves in sieve elements using aphid electrodes.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Salvador-Recatalà, V.
Auteure/Auteur
Tjallingii, W.F.
Auteure/Auteur
Farmer, E.E.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1469-8137
Statut éditorial
Publié
Date de publication
2014
Volume
203
Numéro
2
Première page
674
Dernière page/numéro d’article
684
Langue
anglais
Résumé
Plants propagate electrical signals in response to artificial wounding. However, little is known about the electrophysiological responses of the phloem to wounding, and whether natural damaging stimuli induce propagating electrical signals in this tissue. Here, we used living aphids and the direct current (DC) version of the electrical penetration graph (EPG) to detect changes in the membrane potential of Arabidopsis sieve elements (SEs) during caterpillar wounding. Feeding wounds in the lamina induced fast depolarization waves in the affected leaf, rising to maximum amplitude (c. 60 mV) within 2 s. Major damage to the midvein induced fast and slow depolarization waves in unwounded neighbor leaves, but only slow depolarization waves in non-neighbor leaves. The slow depolarization waves rose to maximum amplitude (c. 30 mV) within 14 s. Expression of a jasmonate-responsive gene was detected in leaves in which SEs displayed fast depolarization waves. No electrical signals were detected in SEs of unwounded neighbor leaves of plants with suppressed expression of GLR3.3 and GLR3.6. EPG applied as a novel approach to plant electrophysiology allows cell-specific, robust, real-time monitoring of early electrophysiological responses in plant cells to damage, and is potentially applicable to a broad range of plant-herbivore interactions.
PID Serval
serval:BIB_DEDD2E6DFACF
PMID
Date de création
2014-08-21T08:34:57.629Z
Date de création dans IRIS
2025-05-21T06:42:32Z