Titre
The optimization of mRNA expression level by its intrinsic properties-Insights from codon usage pattern and structural stability of mRNA.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Victor, M.P.
Auteure/Auteur
Acharya, D.
Auteure/Auteur
Begum, T.
Auteure/Auteur
Ghosh, T.C.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1089-8646
Statut éditorial
Publié
Date de publication
2019-12
Volume
111
Numéro
6
Première page
1292
Dernière page/numéro d’article
1297
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Codon usage bias (CUB) and mRNA structural stability are important intrinsic features of mRNA that correlate positively with mRNA expression level. However, it remains unclear whether the mRNA expression level can be regulated by adjusting these two parameters, influencing the mRNAs' structure. Here we explored the influence of CUB and mRNA structural stability on mRNA expression levels in Saccharomyces cerevisiae, using both wild type and computationally mutated mRNAs. Although in wild type, both CUB and mRNA stability positively regulate the mRNA expression level, any deviation from natural situation breaks such equilibrium. The naturally occurring codon composition is responsible for optimizing the mRNA expression, and under such composition, the mRNA structure having highest stability is selected by nature.
PID Serval
serval:BIB_EC4B2A064C16
PMID
Date de création
2018-09-10T11:44:51.418Z
Date de création dans IRIS
2025-05-21T06:58:21Z