Titre
Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Leftin, A.
Auteure/Auteur
Ben-Chetrit, N.
Auteure/Auteur
Klemm, F.
Auteure/Auteur
Joyce, J.A.
Auteure/Auteur
Koutcher, J.A.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1932-6203
Statut éditorial
Publié
Date de publication
2017
Volume
12
Numéro
9
Première page
e0184765
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
Iron-deposition is a metabolic biomarker of macrophages in both normal and pathological situations, but the presence of iron in tumor and metastasis-associated macrophages is not known. Here we mapped and quantified hemosiderin-laden macrophage (HLM) deposits in murine models of metastatic breast cancer using iron and macrophage histology, and in vivo MRI. Iron MRI detected high-iron pixel clusters in mammary tumors, lung metastasis, and brain metastasis as well as liver and spleen tissue known to contain the HLMs. Iron histology showed these regions to contain clustered macrophages identified by their common iron status and tissue-intrinsic association with other phenotypic macrophage markers. The in vivo MRI and ex vivo histological images were further processed to determine the frequencies and sizes of the iron deposits, and measure the number of HLMs in each deposit to estimate the in vivo MRI sensitivity for these cells. Hemosiderin accumulation is a macrophage biomarker and intrinsic contrast source for cellular MRI associated with the innate function of macrophages in iron metabolism systemically, and in metastatic cancer.
PID Serval
serval:BIB_5D3507371177
PMID
Open Access
Oui
Date de création
2017-09-25T08:29:52.442Z
Date de création dans IRIS
2025-05-20T14:39:48Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
28898277_BIB_5D3507371177.pdf
Version du manuscrit
published
Taille
4.22 MB
Format
Adobe PDF
PID Serval
serval:BIB_5D3507371177.P001
URN
urn:nbn:ch:serval-BIB_5D35073711774
Somme de contrôle
(MD5):5e110c8ac32234090f0e2c9dbb53a464