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  4. Decreased expression of both the alpha1- and alpha2-subunits of the Na-K-ATPase reduces maximal alveolar epithelial fluid clearance.
 
  • Détails
Titre

Decreased expression of both the alpha1- and alpha2-subunits of the Na-K-ATPase reduces maximal alveolar epithelial fluid clearance.

Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
American Journal of Physiology - Lung Cellular and Molecular Physiology  
Auteur(s)
Looney, M.R.
Auteure/Auteur
Sartori, C.
Auteure/Auteur
Chakraborty, S.
Auteure/Auteur
James, P.F.
Auteure/Auteur
Lingrel, J.B.
Auteure/Auteur
Matthay, M.A.
Auteure/Auteur
Liens vers les personnes
Sartori, Claudio  
Liens vers les unités
Service de médecine interne  
ISSN
1040-0605
Statut éditorial
Publié
Date de publication
2005
Volume
289
Numéro
1
Première page
L104
Dernière page/numéro d’article
110
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Résumé
Impaired epithelial sodium channel function predisposes to delayed resorption of pulmonary edema and more severe experimental lung injury, whereas even a small fraction of the normal Na-K-ATPase activity is thought to be sufficient to maintain normal ion transport. However, direct proof is lacking. Therefore, we studied baseline and cAMP stimulated alveolar fluid clearance (AFC) in mice with a 50% decrease in lung protein expression of the alpha(1)- and/or alpha(2)-subunit of the Na-K-ATPase. There was no difference in basal and stimulated AFC in alpha(1)(+/-) or alpha(2)(+/-) mice compared with wild-type littermates. Also, the compound heterozygous mice (alpha(1)(+/-)/alpha(2)(+/-)) had normal basal AFC. However, the combined alpha(1)(+/-)/alpha(2)(+/-) mice showed a significant decrease in cAMP-stimulated AFC compared with wild-type littermates (11.1 +/- 1.0 vs. 14.9 +/- 1.8%/30 min, P < 0.001). When exposed to 96 h of >95% hyperoxia, the decrease in stimulated AFC in the alpha(1)(+/-)/alpha(2)(+/-) mice was not associated with more lung edema compared with wild-type littermates (lung wet-to-dry weight ratio 6.6 +/- 0.9 vs. 5.9 +/- 1.1, respectively; P = not significant). Thus a 50% decrease in protein expression of the alpha(1)- or alpha(2)-subunits of the Na-K-ATPase does not impair basal or stimulated AFC. However, a 50% protein reduction in both the alpha(1)- and alpha(2)-subunits of the Na-K-ATPase produces a submaximal stimulated AFC, suggesting a synergistic role for alpha(1)- and alpha(2)-subunits in cAMP-dependent alveolar epithelial fluid clearance.
Sujets

Animals

Biological Transport,...

Cyclic AMP/metabolism...

Epithelial Cells/enzy...

Extracellular Fluid/m...

Gene Expression Regul...

Hyperoxia/metabolism

Mice

Mice, Knockout

Pulmonary Alveoli/cyt...

Pulmonary Alveoli/met...

Pulmonary Edema/metab...

Sodium-Potassium-Exch...

Sodium-Potassium-Exch...

PID Serval
serval:BIB_3F92B615321A
DOI
10.1152/ajplung.00464.2004
PMID
15781423
WOS
000229772200013
Permalien
https://iris.unil.ch/handle/iris/53398
Date de création
2008-02-22T14:02:33.028Z
Date de création dans IRIS
2025-05-20T14:58:14Z
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