Titre
Phylogenetic characterization of Bifidobacterium kimbladii sp. nov., a novel species from the honey stomach of the honeybee Apis mellifera.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Modesto, M.
Auteure/Auteur
Scarafile, D.
Auteure/Auteur
Vásquez, A.
Auteure/Auteur
Pukall, R.
Auteure/Auteur
Neumann-Schaal, M.
Auteure/Auteur
Pascarelli, S.
Auteure/Auteur
Sgorbati, B.
Auteure/Auteur
Ancora, M.
Auteure/Auteur
Cammà, C.
Auteure/Auteur
Mattarelli, P.
Auteure/Auteur
Olofsson, T.C.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1618-0984
Statut éditorial
Publié
Date de publication
2025-01
Volume
48
Numéro
1
Première page
126579
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Six novel Bifidobacterium strains H1HS16N <sup>T</sup> , Bin2N, Hma3N, H6bp22N, H1HS10N, and H6bp9N, were isolated from the honey stomach of Apis mellifera. Cells are Gram-positive, non-motile, non-sporulating, facultatively anaerobic, and fructose 6-phosphate phosphoketolase-positive. Optimal growth conditions occur at 37 °C in anaerobiosis in MRS medium added with 2 % fructose and 0.1 % L-cysteine. The 16S rRNA gene sequences analysis revealed clustering with Bifidobacterium species found in honeybees. Strains Hma3N, H6bp22N, and H1HS16N <sup>T</sup> showed significant similarity to Bifidobacterium polysaccharolyticum JCM 34588 <sup>T</sup> , with an average similarity of 99.63 %. In contrast, strains Bin2N, H1HS10N, and H6bp9N were closely related to Bifidobacterium apousia JCM 34587 <sup>T</sup> , with an average similarity of 99.22 %. Moreover, strains Hma3N and H6bp22N exhibited ANI values of 96.65 % and 96.53 % when compared to Bifidobacterium polysaccharolyticum JCM 34588 <sup>T</sup> , while strains H1HS16N <sup>T</sup> , Bin2N, H6bp9N, and H1HS10N revealed ANI values of 94.18 %, 94.33 %, 94.22 %, and 95.50 % respectively when compared to B. apousia JCM 34587 <sup>T</sup> . dDDH analysis confirmed that strains Hma3N and H6bp22N belong to B. polysaccharolyticum, whereas strains H1HS16N <sup>T</sup> , Bin2N, H6bp9N, and H1HS10N represent a novel species. The peptidoglycan of the novel species is of the A4α type (L-Lys-D-Asp). The main cellular fatty acids of the type strain H1HS16N <sup>T</sup> are C <sub>16:0</sub> , C <sub>14:0</sub> , C <sub>19:0</sub> cyclo ω9c, and C <sub>18:1</sub> ω9c. The DNA G + C content of the type strain is 60.8 mol%. Genome analyses of the strains were also conducted to determine their biosynthesis-related gene clusters, probiotic features, and ecological distribution patterns. Phenotypic and genotypic characterization show that strain H1HS16N <sup>T</sup> is distinct from the type strains of other recognized Bifidobacterium species. Thus, Bifidobacterium kimbladii sp. nov. (H1HS16N <sup>T</sup> = DSM 115187 <sup>T</sup> = CCUG 76695 <sup>T</sup> ) is proposed as a novel Bifidobacterium species.
Sujets
PID Serval
serval:BIB_1C5366735A3D
PMID
Date de création
2025-01-10T14:10:20.346Z
Date de création dans IRIS
2025-05-20T15:17:41Z