Titre
N-Nitrosation Based Fluorescence Turn-On Nitric Oxide Probe: Kinetic and Cell Imaging Studies.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Dutta, A.
Auteure/Auteur
Maiti, D.
Auteure/Auteur
Katarkar, A.
Auteure/Auteur
Sasmal, M.
Auteure/Auteur
Khatun, R.
Auteure/Auteur
Moni, D.
Auteure/Auteur
Habibullah, M.
Auteure/Auteur
Ali, M.
Auteure/Auteur
Liens vers les unités
ISSN
2576-6422
Statut éditorial
Publié
Date de publication
2023-08-21
Volume
6
Numéro
8
Première page
3266
Dernière page/numéro d’article
3277
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Nitric oxide (NO) is a ubiquitous messenger molecule playing a key role in various physiological and pathological processes. However, producing a selective turn-on fluorescence response to NO is a challenging task due to (a) the very short half-life of NO (typically in the range of 0.1-10 s) in the biological milieu and (b) false positive responses to reactive carbonyl species (RCS) (e.g., dehydroascorbic acid and methylglyoxal etc.) and some other reactive oxygen/nitrogen species (ROS/RNS), especially with o-phenylenediamine (OPD) based fluorosensors. To avoid these limitations, NO sensors should be designed in such a way that they react spontaneously with NO to give turn-on response within the time frame of t <sub>1/2</sub> (typically in the range of 0.1-10 s) of NO and λ <sub>em</sub> in the visible wavelength along with good cell permeability to achieve biocompatibility. With these views in mind, a N-nitrosation based fluorescent sensor, NDAQ, has been developed that is highly selective to NO with ∼27-fold fluorescence enhancement at λ <sub>em</sub> = 542 nm with high sensitivity (LOD = 7 ± 0.4 nM) and shorter response time, eliminating the interference of other reactive species (RCS/ROS/RNS). Furthermore, all the photophysical studies with NDAQ have been performed in 98% aqueous medium at physiological pH, indicating its good stability under physiological conditions. The kinetic assay illustrates the second-order dependency with respect to NO concentration and first-order dependency with respect to NDAQ concentration. The biological studies reveal the successful application of the probe to track both endogenous and exogenous NO in living organisms.
PID Serval
serval:BIB_40F2CB4CCC11
PMID
Date de création
2023-08-10T12:24:26.188Z
Date de création dans IRIS
2025-05-20T15:35:49Z