Titre
Experimental Models of Liquid Biopsy in Hepatocellular Carcinoma Reveal Clone-Dependent Release of Circulating Tumor DNA.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Labgaa, I.
Auteure/Auteur
von Felden, J.
Auteure/Auteur
Craig, A.J.
Auteure/Auteur
Martins-Filho, S.N.
Auteure/Auteur
Villacorta-Martin, C.
Auteure/Auteur
Demartines, N.
Auteure/Auteur
Dormond, O.
Auteure/Auteur
D'Avola, D.
Auteure/Auteur
Villanueva, A.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2471-254X
Statut éditorial
Publié
Date de publication
2021-06
Volume
5
Numéro
6
Première page
1095
Dernière page/numéro d’article
1105
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
Liquid biopsy, the molecular analysis of tumor components released into the bloodstream, has emerged as a noninvasive and resourceful means to access genomic information from cancers. Most data derived from translational studies showcase its numerous potential clinical applications. However, data from experimental models are scarce, and little is known about the underlying mechanisms and factors controlling the release of circulating tumor DNA (ctDNA) and cells (CTCs). This study aimed to model liquid biopsy in hepatocellular carcinoma xenografts and to study the dynamics of release of ctDNA and CTCs; this included models of intratumoral heterogeneity (ITH) and metastatic disease. We quantified ctDNA by quantitative polymerase chain reaction (PCR) targeting human long interspersed nuclear element group 1; targeted mutation analysis was performed with digital droplet PCR. CTCs were traced by flow cytometry. Results demonstrated the feasibility of detecting ctDNA, including clone-specific mutations, as well as CTCs in blood samples of mice. In addition, the concentration of ctDNA and presence of tumor-specific mutations reflected tumor progression, and detection of CTCs was associated with metastases. Our ITH model suggested differences in the release of DNA fragments impacted by the cell-clone origin and the treatment. Conclusion: These data present new models to study liquid biopsy and its underlying mechanisms and highlighted a clone-dependent release of ctDNA into the bloodstream.
PID Serval
serval:BIB_337546F85B24
PMID
Open Access
Oui
Date de création
2021-04-03T07:29:20.570Z
Date de création dans IRIS
2025-05-20T16:26:31Z
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Nom
34141992_BIB_337546F85B24.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by-nc-nd/4.0
Taille
1.03 MB
Format
Adobe PDF
PID Serval
serval:BIB_337546F85B24.P001
URN
urn:nbn:ch:serval-BIB_337546F85B242
Somme de contrôle
(MD5):3c07f635e54efc980aa32ff89fb22544