Titre
Vasoactive intestinal peptide, pituitary adenylate cyclase-activating peptide, and noradrenaline induce the transcription factors CCAAT/enhancer binding protein (C/EBP)-beta and C/EBP delta in mouse cortical astrocytes: involvement in cAMP-regulated glycogen metabolism.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Cardinaux, J.R.
Auteure/Auteur
Magistretti, P.J.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0270-6474
Statut éditorial
Publié
Date de publication
1996
Volume
16
Numéro
3
Première page
919
Dernière page/numéro d’article
929
Peer-reviewed
Oui
Langue
anglais
Résumé
We have described previously a transcription-dependent induction of glycogen resynthesis by the vasoactive intestinal peptide (VIP) or noradrenaline (NA) in astrocytes, which is mediated by cAMP. Because it has been postulated that the cAMP-mediated regulation of energy balance in hepatocytes and adipocytes is channeled at least in part through the CCAAT/enhancer binding protein (C/EBP) family of transcription factors, we tested the hypothesis that C/EBP isoforms could be expressed in mouse cortical astrocytes and that their level of expression could be regulated by VIP, by the VIP-related neuropeptide pituitary adenylate cyclase-activating peptide (PACAP), or by NA. We report in this study that in these cells, C/EBP beta and C/EBP delta are induced by VIP, PACAP, or NA via the cAMP second-messenger pathway. Induction of C/EBP beta and -delta mRNA by VIP occurs in the presence of a protein synthesis inhibitor. Thus, c/ebp beta and c/ebp delta behave as cAMP-inducible immediate-early genes in astrocytes. Moreover, transfection of astrocytes with expression vectors selectively producing the transcriptionally active form of C/EBP beta, termed liver-enriched transcriptional activator protein, or C/EBP delta enhance the glycogen resynthesis elicited by NA, whereas an expression vector producing the transcriptionally inactive form of C/EBP beta, termed liver-enriched transcriptional inhibitory protein, reduces this resynthesis. These results support the idea that C/EBP beta and -delta regulate gene expression of energy metabolism-related enzymes in astrocytes.
Sujets
PID Serval
serval:BIB_2A9ED0B306D3
PMID
Date de création
2008-02-18T09:32:04.213Z
Date de création dans IRIS
2025-05-20T16:48:52Z
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Nom
919.full.pdf
Version du manuscrit
published
Taille
5.94 MB
Format
Adobe PDF
PID Serval
serval:BIB_2A9ED0B306D3.P001
URN
urn:nbn:ch:serval-BIB_2A9ED0B306D35
Somme de contrôle
(MD5):b934afc9408d684a08ae45621dc950cf