Titre
Quantification, self-renewal, and genetic tracing of FL1+ tumor-initiating cells in a large cohort of human gliomas.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Clément-Schatlo, V.
Auteure/Auteur
Marino, D.
Auteure/Auteur
Burkhardt, K.
Auteure/Auteur
Teta, P.
Auteure/Auteur
Leyvraz, F.
Auteure/Auteur
Schatlo, B.
Auteure/Auteur
Frank, S.
Auteure/Auteur
Schaller, K.
Auteure/Auteur
Castella, V.
Auteure/Auteur
Radovanovic, I.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1523-5866
Statut éditorial
Publié
Date de publication
2012-06
Volume
14
Numéro
6
Première page
720
Dernière page/numéro d’article
735
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
Evidence has emerged that the initiation and growth of gliomas is sustained by a subpopulation of cancer-initiating cells (CICs). Because of the difficulty of using markers to tag CICs in gliomas, we have previously exploited more robust phenotypic characteristics, including a specific morphology and intrincic autofluorescence, to identify and isolate a subpopulation of glioma CICs, called FL1(+). The objective of this study was to further validate our method in a large cohort of human glioma and a mouse model of glioma. Seventy-four human gliomas of all grades and the GFAP-V(12)HA-ras B8 mouse model were analyzed for in vitro self-renewal capacity and their content of FL1(+). Nonneoplastic brain tissue and embryonic mouse brain were used as control. Genetic traceability along passages was assessed with microsatellite analysis. We found that FL1(+) cells from low-grade gliomas and from control nonneoplasic brain tissue show a lower level of autofluorescence and undergo a restricted number of cell divisions before dying in culture. In contrast, we found that FL1(+) cells derived from many but not all high-grade gliomas acquire high levels of autofluorescence and can be propagated in long-term cultures. Moreover, FL1(+) cells show a remarkable traceability over time in vitro and in vivo. Our results show that FL1(+) cells can be found in all specimens of a large cohort of human gliomas of different grades and in a model of genetically induced mouse glioma as well as nonneoplastic brain. However, their self-renewal capacity is variable and seems to be dependent on the tumor grade.
PID Serval
serval:BIB_25D2B86A387D
PMID
Open Access
Oui
Date de création
2012-06-29T16:33:24.318Z
Date de création dans IRIS
2025-05-20T17:25:53Z