Titre
Adult-derived human liver mesenchymal-like cells as a potential progenitor reservoir of hepatocytes?
Type
article
Institution
Externe
Périodique
Auteur(s)
Najimi, M.
Auteure/Auteur
Khuu, D.N.
Auteure/Auteur
Lysy, P.A.
Auteure/Auteur
Jazouli, N.
Auteure/Auteur
Abarca, J.
Auteure/Auteur
Sempoux, C.
Auteure/Auteur
Sokal, E.M.
Auteure/Auteur
Liens vers les personnes
ISSN
0963-6897
Statut éditorial
Publié
Date de publication
2007
Volume
16
Numéro
7
Première page
717
Dernière page/numéro d’article
728
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
It is currently accepted that adult tissues may develop and maintain their own stem cell pools. Because of their higher safety profile, adult stem cells may represent an ideal candidate cell source to be used for liver cell therapies. We therefore evaluated the differentiation potential of mesenchymal-like cells isolated from adult human livers. Mesenchymal-like cells were isolated from enzymatically digested adult human liver and expanded in vitro. Cell characterization was performed using flow cytometry, RT-PCR, and immunofluorescence, whereas the differentiation potential was evaluated both in vitro after incubation with specific media and in vivo after intrasplenic transplantation of uPA(+/+)-SCID and SCID mice. Adult-derived human liver mesenchymal-like cells expressed both hepatic and mesenchymal markers among which albumin, CYP3A4, vimentin, and alpha-smooth muscle actin. In vitro differentiation studies demonstrated that these mesenchymal-like cells are preferentially determined to differentiate into hepatocyte-like cells. Ten weeks following intrasplenic transplantation into uPA(+/+)-SCID mice, recipient livers showed the presence of human hepatocytic cell nodules positive for human albumin, prealbumin, and alpha-fetoprotein. In SCID transplanted liver mice, human hepatocyte-like cells were mostly found near vascular structures 56 days posttransplantation. In conclusion, the ability of isolated adult-derived liver mesenchymal stem-like cells to proliferate and differentiate into hepatocyte-like cells both in vitro and in vivo leads to propose them as an attractive expandable cell source for stem cell therapy in human liver diseases.
PID Serval
serval:BIB_0FCD43D65451
PMID
Date de création
2016-10-20T15:09:09.390Z
Date de création dans IRIS
2025-05-20T17:43:06Z
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Nom
18019361.pdf
Version du manuscrit
published
Taille
1.78 MB
Format
Adobe PDF
PID Serval
serval:BIB_0FCD43D65451.P001
Somme de contrôle
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